Aminoglycoside resistance profile and structural architecture of the aminoglycoside acetyltransferase AAC(6')-Im.

Autor:	Smith CA; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Bhattacharya M; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA., Toth M; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA., Stewart NK; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA., Vakulenko SB; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA.
Jazyk:	angličtina
Zdroj:	Microbial cell (Graz, Austria) [Microb Cell] 2017 Nov 09; Vol. 4 (12), pp. 402-410. Date of Electronic Publication: 2017 Nov 09.
DOI:	10.15698/mic2017.12.602
Abstrakt:	Aminoglycoside 6'-acetyltransferase-Im (AAC(6')-Im) is the closest monofunctional homolog of the AAC(6')-Ie acetyltransferase of the bifunctional enzyme AAC(6')-Ie/APH(2")-Ia. The AAC(6')-Im acetyltransferase confers 4- to 64-fold higher MICs to 4,6-disubstituted aminoglycosides and the 4,5-disubstituted aminoglycoside neomycin than AAC(6')-Ie, yet unlike AAC(6')-Ie, the AAC(6')-Im enzyme does not confer resistance to the atypical aminoglycoside fortimicin. The structure of the kanamycin A complex of AAC(6')-Im shows that the substrate binds in a shallow positively-charged pocket, with the N6' amino group positioned appropriately for an efficient nucleophilic attack on an acetyl-CoA cofactor. The AAC(6')-Ie enzyme binds kanamycin A in a sufficiently different manner to position the N6' group less efficiently, thereby reducing the activity of this enzyme towards the 4,6-disubstituted aminoglycosides. Conversely, docking studies with fortimicin in both acetyltransferases suggest that the atypical aminoglycoside might bind less productively in AAC(6')-Im, thus explaining the lack of resistance to this molecule. Competing Interests: Conflict of interest: The authors declare no conflict of interest.
Databáze:	MEDLINE
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