Application of MALDI-TOF/TOF-MS for relative quantitation of α- and β-Asp7 isoforms of amyloid-β peptide.

Autor:	Pekov S; 1 65014 Moscow Institute of Physics and Technology , Moscow, Russia.; 2 V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia., Indeykina M; 2 V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.; 3 Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia., Popov I; 1 65014 Moscow Institute of Physics and Technology , Moscow, Russia.; 2 V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia., Kononikhin A; 1 65014 Moscow Institute of Physics and Technology , Moscow, Russia.; 2 V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia., Bocharov K; 1 65014 Moscow Institute of Physics and Technology , Moscow, Russia.; 2 V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia., Kozin SA; 5 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Makarov AA; 5 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Nikolaev E; 2 V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.; 3 Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.; 4 Skolkovo Institute of Science and Technology, Skolkovo, Russia.
Jazyk:	angličtina
Zdroj:	European journal of mass spectrometry (Chichester, England) [Eur J Mass Spectrom (Chichester)] 2018 Feb; Vol. 24 (1), pp. 141-144. Date of Electronic Publication: 2017 Sep 08.
DOI:	10.1177/1469066717730544
Abstrakt:	It is known that aspartic acid isomerization process plays a role in aging processes and may be used as a marker for aging of natural materials. As for Alzheimer's disease, the most abundant modification in the peptide profile is the aspartate isomerization of amyloid-β. Liquid chromatography-electrospray ionization-mass spectrometry/mass spectrometry-based approaches with Collision Induced Dissociation (CID) or Electron Capture Dissociation (ECD) fragmentation provide a good and precise method for the relative quantitation of iso- to normal amyloid-β peptides but require additional time consuming steps. In this study, MALDI-TOF/TOF-matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry (MS) method was developed as a high-throughput approach for the relative quantitation of the isomerized form of the amyloid-β peptide.
Databáze:	MEDLINE
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