Epitope-Resolved Detection of Peanut-Specific IgE Antibodies by Surface Plasmon Resonance Imaging.

Autor: Shen M; Department of Chemistry, University of Connecticut, Storrs, CT, 06269, USA., Joshi AA; Department of Chemistry, University of Connecticut, Storrs, CT, 06269, USA., Vannam R; Department of Chemistry, University of Connecticut, Storrs, CT, 06269, USA., Dixit CK; Department of Chemistry, University of Connecticut, Storrs, CT, 06269, USA., Hamilton RG; Division of Allergy and Clinical Immunology, Johns Hopkins University School of Medicine, Baltimore, MD, 21224, USA., Kumar CV; Department of Chemistry, University of Connecticut, Storrs, CT, 06269, USA.; Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 06269, USA., Rusling JF; Department of Chemistry, University of Connecticut, Storrs, CT, 06269, USA.; Department of Cell Biology, University of Connecticut Health Center, Farmington, CT, 06032, USA., Peczuh MW; Department of Chemistry, University of Connecticut, Storrs, CT, 06269, USA.
Jazyk: angličtina
Zdroj: Chembiochem : a European journal of chemical biology [Chembiochem] 2018 Feb 02; Vol. 19 (3), pp. 199-202. Date of Electronic Publication: 2018 Jan 04.
DOI: 10.1002/cbic.201700513
Abstrakt: Peanut allergy can be life-threatening and is mediated by allergen-specific immunoglobulin E (IgE) antibodies. Investigation of IgE antibody binding to allergenic epitopes can identify specific interactions underlying the allergic response. Here, we report a surface plasmon resonance imaging (SPRi) immunoassay for differentiating IgE antibodies by epitope-resolved detection. IgE antibodies were first captured by magnetic beads bearing IgE ϵ-chain-specific antibodies and then introduced into an SPRi array immobilized with epitopes from the major peanut allergen glycoprotein Arachis hypogaea h2 (Ara h2). Differential epitope responses were achieved by establishing a binding environment that minimized cross-reactivity while maximizing analytical sensitivity. IgE antibody binding to each Ara h2 epitope was distinguished and quantified from patient serum samples (10 μL each) in a 45 min assay. Excellent correlation of Ara h2-specific IgE values was found between ImmunoCAP assays and the new SPRi method.
(© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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