| Autor: |
Wu J; Department of Chemistry and Biochemistry, The Ohio State University , Columbus, Ohio 43210, United States., Sabag-Daigle A; Department of Microbial Infection and Immunity, The Ohio State University , Columbus, Ohio 43210, United States., Metz TO; Biological Sciences Division, Pacific Northwest National Laboratory , Richland 99352, Washington, United States., Deatherage Kaiser BL; Signature Sciences and Technology Division, Pacific Northwest National Laboratory , Richland 99352, Washington, United States., Gopalan V; Department of Chemistry and Biochemistry, The Ohio State University , Columbus, Ohio 43210, United States., Behrman EJ; Department of Chemistry and Biochemistry, The Ohio State University , Columbus, Ohio 43210, United States., Wysocki VH; Department of Chemistry and Biochemistry, The Ohio State University , Columbus, Ohio 43210, United States., Ahmer BMM; Department of Microbial Infection and Immunity, The Ohio State University , Columbus, Ohio 43210, United States. |
| Abstrakt: |
The food-borne bacterial pathogen, Salmonella enterica, can utilize fructose-asparagine (F-Asn) as its sole carbon and nitrogen source. F-Asn is the product of an Amadori rearrangement following the nonenzymatic condensation of glucose and asparagine. Heating converts F-Asn via complex Maillard reactions to a variety of molecules that contribute to the color, taste, and aroma of heated foods. Among these end derivatives is acrylamide, which is present in some foods, especially in fried potatoes. The F-Asn utilization pathway in Salmonella, specifically FraB, is a potential drug target because inhibition of this enzyme would lead to intoxication of Salmonella in the presence of F-Asn. However, F-Asn would need to be packaged with the FraB inhibitor or available in human foods. To determine if there are foods that have sufficient F-Asn, we measured F-Asn concentrations in a variety of human and animal foods. The 400 pmol/mg F-Asn found in mouse chow is sufficient to intoxicate a Salmonella fraB mutant in mouse models of salmonellosis, and several human foods were found to have F-Asn at this level or higher (fresh apricots, lettuce, asparagus, and canned peaches). Much higher concentrations (11 000-35 000 pmol/mg dry weight) were found in heat-dried apricots, apples, and asparagus. This report reveals possible origins of F-Asn as a nutrient source for Salmonella and identifies foods that could be used together with a FraB inhibitor as a therapeutic agent for Salmonella. |
