Gene expression profiling in the intestinal mucosa of obese rats administered probiotic bacteria.

Autor: Plaza-Díaz J; Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, Granada 18071, Spain.; Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Center, Parque Tecnológico Ciencias de la Salud, University of Granada, Granada 18016, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain., Robles-Sánchez C; Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, Granada 18071, Spain.; Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Center, Parque Tecnológico Ciencias de la Salud, University of Granada, Granada 18016, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain., Abadía-Molina F; Department of Cell Biology, School of Sciences, University of Granada, Granada 18071, Spain., Sáez-Lara MJ; Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Center, Parque Tecnológico Ciencias de la Salud, University of Granada, Granada 18016, Spain.; Department of Biochemistry and Molecular Biology I, School of Sciences, University of Granada, Granada 18071, Spain., Vilchez-Padial LM; Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Center, Parque Tecnológico Ciencias de la Salud, University of Granada, Granada 18016, Spain., Gil Á; Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, Granada 18071, Spain.; Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Center, Parque Tecnológico Ciencias de la Salud, University of Granada, Granada 18016, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain.; CIBEROBN, Instituto de Salud Carlos III, Madrid 28029, Spain., Gómez-Llorente C; Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, Granada 18071, Spain.; Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Center, Parque Tecnológico Ciencias de la Salud, University of Granada, Granada 18016, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain.; CIBEROBN, Instituto de Salud Carlos III, Madrid 28029, Spain., Fontana L; Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, Granada 18071, Spain.; Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Center, Parque Tecnológico Ciencias de la Salud, University of Granada, Granada 18016, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain.
Jazyk: angličtina
Zdroj: Scientific data [Sci Data] 2017 Dec 12; Vol. 4, pp. 170186. Date of Electronic Publication: 2017 Dec 12.
DOI: 10.1038/sdata.2017.186
Abstrakt: We investigated whether the administration of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 modulate the expression of genes in the intestinal mucosa of obese Zucker rats. Forty-eight Zucker-Lepr fa/fa and 16 Zucker lean Lepr +/fa rats were used. Eight Zucker lean Lepr +/fa and 8 Zucker-Lepr fa/fa rats were euthanized as a reference. The remaining 40 Zucker-Lepr fa/fa rats were then assigned to receive 10 10 colony forming units (CFU) of one of the three probiotic strains, a mixture of L. paracasei CNCM I-4034 and B. breve CNCM I-4035, or a placebo by oral administration for 30 days. An additional group of 8 Zucker lean Lepr +/fa rats received the placebo for 30 days. Over 27,000 rat genes were studied using a DNA array. Four animals per group were used. Total RNA was extracted from intestinal mucosa and cDNA was synthesized, fragmented and labeled. Labeled cDNA was hybridized using GeneChip kits, and the latter were scanned. Intensity values of each probe were processed and normalized to obtain an individual value for each set of probes.
Databáze: MEDLINE
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