Non-functionalized soft alginate hydrogel promotes locomotor recovery after spinal cord injury in a rat hemimyelonectomy model.

Autor: Sitoci-Ficici KH; Neurosurgery, University Hospital Carl Gustav Carus, TU Dresden, Dresden, Germany., Matyash M; Neurosurgery, University Hospital Carl Gustav Carus, TU Dresden, Dresden, Germany.; Neuropediatrics, University Hospital Carl Gustav Carus, TU Dresden, Dresden, Germany., Uckermann O; Neurosurgery, University Hospital Carl Gustav Carus, TU Dresden, Dresden, Germany., Galli R; Clinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, TU Dresden, Dresden, Germany., Leipnitz E; Neurosurgery, University Hospital Carl Gustav Carus, TU Dresden, Dresden, Germany., Later R; Neurosurgery, University Hospital Carl Gustav Carus, TU Dresden, Dresden, Germany., Ikonomidou C; Neuropediatrics, University Hospital Carl Gustav Carus, TU Dresden, Dresden, Germany.; Department of Neurology, Developmental Brain Injury Laboratory, University of Wisconsin, Madison, WI, USA., Gelinsky M; Center for Translational Bone, Joint and Soft Tissue Research, TU Dresden, Dresden, Germany.; CRTD/DFG-Center for Regenerative Therapies Dresden-Cluster of Excellence, TU Dresden, Dresden, Germany., Schackert G; Neurosurgery, University Hospital Carl Gustav Carus, TU Dresden, Dresden, Germany., Kirsch M; Neurosurgery, University Hospital Carl Gustav Carus, TU Dresden, Dresden, Germany. matthias.kirsch@uniklinikum-dresden.de.; CRTD/DFG-Center for Regenerative Therapies Dresden-Cluster of Excellence, TU Dresden, Dresden, Germany. matthias.kirsch@uniklinikum-dresden.de.
Jazyk: angličtina
Zdroj: Acta neurochirurgica [Acta Neurochir (Wien)] 2018 Mar; Vol. 160 (3), pp. 449-457. Date of Electronic Publication: 2017 Dec 11.
DOI: 10.1007/s00701-017-3389-4
Abstrakt: Background: Spinal cord injury (SCI) and the consecutive devastating neurological sequelae have an enormous individual and economic impact. Implantation of functionalized hydrogels is a promising approach, because they can serve as a matrix for the regenerating tissue, carry and release bioactive molecules and various cell types. We already demonstrated that non-functionalized soft alginate hydrogel supported axonal outgrowth and protected neurons against oxidative stress in vitro. Here, we investigated the effects of such soft alginate hydrogels on locomotor recovery in small and large spinal cord lesions.
Method: Hemimyelonectomy of 2 mm or 4 mm length was performed in rats and soft alginate hydrogel was implanted. Functional recovery of the hindlimbs was assessed in the open field [Batto Beattie Bresnahan (BBB) score] and using swimming test [Louisville Swim score (LSS)] for 140 days post injury (DPI). Reference histology was performed.
Results: Rats that received an alginate implant into 2 mm spinal cord lesions demonstrated significantly improved locomotor recovery compared to controls detectable already at 10 DPI. At 140 DPI, they reached higher LSS and BBB scores in swimming and open field tests, respectively. However, this beneficial effect of alginate was lacking in animals with larger (4 mm) lesions. Histological examination suggested that fibrous scarring in the spinal cord was reduced after alginate implantation in comparison to controls.
Conclusions: Implantation of soft alginate hydrogel in small spinal cord lesions improved functional recovery. Possible underlying mechanisms include the mechanical stabilization of the wound, reduction of secondary damage and inhibition of fibrous scarring.
Databáze: MEDLINE
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