| Autor: |
Sedlačík T; Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Department of Biomaterials and Bioanalogous Systems, Heyrovsky sq. 2, 162 06 - Prague 6, Czech Republic. sedlacik@imc.cas.cz., K Acar O, Studenovská H, Kotelnikov I, Kučka J, Konečná Z, Zikmund T, Kaiser J, Kose GT, Rypáček F |
| Jazyk: |
angličtina |
| Zdroj: |
Soft matter [Soft Matter] 2018 Jan 03; Vol. 14 (2), pp. 228-238. |
| DOI: |
10.1039/c7sm02074k |
| Abstrakt: |
In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(α-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N 5 -(2-hydroxyethyl)-l-glutamine-stat-N 5 -(2-methacryloyl-oxy-ethyl)-l-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers. PrMAAm provided alkyne groups for modifying the gels with cell-supporting moieties (RGDS peptides) by the azide-alkyne "click"-reaction. Two types of gels with different compressive moduli were prepared. Each type was modified with two different concentrations of RGDS peptide. X-ray computed nanotomography (nanoCT) was used to visualize and analyze the 3D-structure of the cryogels. It was shown that modifying the PHEG-MA cryogels within the range of RGDS concentrations examined here had a positive effect on the proliferation of hDPSCs. Immunofluorescence staining for collagen type 2 and aggrecan proved that there was differentiation of hDPSCs into chondrocytes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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