Concentration dependence of the cell membrane permeability to cryoprotectant and water and implications for design of methods for post-thaw washing of human erythrocytes.

Autor: Lahmann JM; School of Chemical, Biological and Environmental Engineering, Oregon State University, United States., Benson JD; Department of Biology, University of Saskatchewan, Canada., Higgins AZ; School of Chemical, Biological and Environmental Engineering, Oregon State University, United States. Electronic address: adam.higgins@oregonstate.edu.
Jazyk: angličtina
Zdroj: Cryobiology [Cryobiology] 2018 Feb; Vol. 80, pp. 1-11. Date of Electronic Publication: 2017 Dec 06.
DOI: 10.1016/j.cryobiol.2017.12.003
Abstrakt: For more than fifty years the human red blood cell (RBC) has been a widely studied model for transmembrane mass transport. Existing literature spans myriad experimental designs with varying results and physiologic interpretations. In this review, we examine the kinetics and mechanisms of membrane transport in the context of RBC cryopreservation. We include a discussion of the pathways for water and glycerol permeation through the cell membrane and the implications for mathematical modeling of the membrane transport process. In particular, we examine the concentration dependence of water and glycerol transport and provide equations for estimating permeability parameters as a function of concentration based on a synthesis of literature data. This concentration-dependent transport model may allow for design of improved methods for post-thaw removal of glycerol from cryopreserved blood. More broadly, the consideration of the concentration dependence of membrane permeability parameters may be important for other cell types as well, especially for design of methods for equilibration with the highly concentrated solutions used for vitrification.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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