Novel in vitro and in silico insights of the multi-biological activities and chemical composition of Bidens tripartita L.

Autor: Uysal S; Selcuk University, Science Faculty, Department of Biology, Campus, 42250, Konya, Turkey. Electronic address: sennguluysal@gmail.com., Ugurlu A; Department of Biology, Faculty of Science and Arts, Kastamonu University, Kastamonu, Turkey. Electronic address: a.z.ugurlu@gmail.com., Zengin G; Selcuk University, Science Faculty, Department of Biology, Campus, 42250, Konya, Turkey., Baloglu MC; Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu University, Kastamonu, Turkey., Altunoglu YC; Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu University, Kastamonu, Turkey., Mollica A; Department of Pharmacy University 'G. d'Annunzio' of Chieti-Pescara, 66100, Chieti, Italy., Custodio L; Centre of Marine Sciences, Faculty of Sciences and Technology, Campus of Gambelas, University of Algarve, Faro, Portugal., Neng NR; Faculty of Sciences of the University of Lisbon, Centre of Chemistry and Biochemistry/Department of Chemistry and Biochemistry, Building C8, Floor 5, Campo Grande, 1749-016 Lisbon, Portugal., Nogueira JMF; Faculty of Sciences of the University of Lisbon, Centre of Chemistry and Biochemistry/Department of Chemistry and Biochemistry, Building C8, Floor 5, Campo Grande, 1749-016 Lisbon, Portugal., Mahomoodally MF; Department of Health Sciences, Faculty of Science, University of Mauritius, Réduit, Mauritius.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2018 Jan; Vol. 111, pp. 525-536. Date of Electronic Publication: 2017 Dec 05.
DOI: 10.1016/j.fct.2017.11.058
Abstrakt: Bidens tripartita L. is a traditional phyto-remedy used in several countries, yet there is still a paucity of data on its biological potential. We aimed to provide new insights on the pharmacological potential of extracts prepared from B. tripartita via highlighting its antioxidant, key enzymes inhibitory potency, and DNA protecting effects. Phytochemical profile was established using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) and bioactive compound(s) docked against target enzymes using in silico methods. Cytotoxicity against three cancer cell lines was assessed using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) cell viability test. The main compounds were luteolin-7-glucoside (cynaroside), chlorogenic acid, and epicatechin in the extracts. The methanol extract exhibited the highest radical scavenging activity. Ethyl acetate extract showed strongest α-amylase inhibitory activity, while the best α-glucosidase inhibitory effect recorded for the methanol extract. Molecular docking showed that cynaroside strongly interact to α-glucosidase cavity by establishing six hydrogen bonds. B. tripartita extracts were found to protect supercoiled form of pUC19 plasmid (>70%) and also showed anti-proliferative properties. Results amassed in the present study add on to a growing body of literature on the multi-pharmacological potency of B. tripartita which can be applied to bio-products development geared towards management of common diseases.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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