In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization.

Autor:	Vieira AEM; Univ. Estadual Paulista, Faculdade de Odontologia de Araçatuba, Departamento de Odontologia Infantil e Social, Araçatuba, SP, Brasil., Danelon M; Univ. Estadual Paulista, Faculdade de Odontologia de Araçatuba, Departamento de Odontologia Infantil e Social, Araçatuba, SP, Brasil., Camara DMD; Univ. Estadual Paulista, Faculdade de Odontologia de Araçatuba, Departamento de Odontologia Infantil e Social, Araçatuba, SP, Brasil., Rosselli ER; Univ. Estadual Paulista, Faculdade de Odontologia de Araçatuba, Departamento de Odontologia Infantil e Social, Araçatuba, SP, Brasil., Stock SR; Northwestern University, Feinberg School of Medicine, Department of Molecular Pharmacology and Biological Chemistry, Chicago, IL USA., Cannon ML; Northwestern University, Feinberg School of Medicine, Ann and Robert Lurie Children's Hospital, Chicago, IL, USA., Xiao X; Argonne National Laboratory, Advanced Photon Source, Argonne, IL, USA., De Carlo F; Argonne National Laboratory, Advanced Photon Source, Argonne, IL, USA., Delbem ACB; Univ. Estadual Paulista, Faculdade de Odontologia de Araçatuba, Departamento de Odontologia Infantil e Social, Araçatuba, SP, Brasil.
Jazyk:	angličtina
Zdroj:	Journal of applied oral science : revista FOB [J Appl Oral Sci] 2017 Nov-Dec; Vol. 25 (6), pp. 596-603.
DOI:	10.1590/1678-7757-2016-0513
Abstrakt:	Objectives: Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm-3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.
Databáze:	MEDLINE
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