| Autor: |
Scarpelli BB; Department of Paediatric Dentistry, UEL - Universidade Estadual de Londrina, Londrina, PR, Brazil.; Department of Restorative Dentistry, UNOPAR - Universidade do Norte do Paraná, Londrina, PR, Brazil., Punhagui MF; Department of Paediatric Dentistry, UEL - Universidade Estadual de Londrina, Londrina, PR, Brazil.; Department of Restorative Dentistry, UNOPAR - Universidade do Norte do Paraná, Londrina, PR, Brazil., Hoeppner MG; Department of Restorative Dentistry, UEL - Universidade Estadual de Londrina, Londrina, PR, Brazil., Almeida RSC; Department of Microbiology, UEL - Universidade Estadual de Londrina, Londrina, PR, Brazil., Juliani FA; Department of Microbiology, UEL - Universidade Estadual de Londrina, Londrina, PR, Brazil., Guiraldo RD; Department of Restorative Dentistry, UNOPAR - Universidade do Norte do Paraná, Londrina, PR, Brazil., Berger SB; Department of Restorative Dentistry, UNOPAR - Universidade do Norte do Paraná, Londrina, PR, Brazil. |
| Abstrakt: |
Cariostatic treatment has been shown to successfully arrest caries. However, it blackens the carious tooth structure. This study evaluated the effects of an experimental cariostatic agent with silver nanoparticles (Ag-Nano) using microhardness (MH) and microbiological tests. The cariostatic agents tested were: Saforide®, Cariestop®, Ancarie® and Ag-Nano. Sixty-six samples from deciduous enamel were submitted to initial (after pH cycling to obtain initial caries-like lesion) and final (after cariostatic application) MH testing and %MH values were calculated. After longitudinal sectioning, internal (I) MH was evaluated. Strains of Streptococcus mutans, Escherichia coli, and Enterococcus faecalis in brain-heart infusion culture were treated with the cariostatic agents. Agar diffusion tests (ADTs) were performed and minimum inhibitory concentrations were determined. The statistical tests used were: Kruskal-Wallis and Dunn (%MD; ADT; MIC) and ANOVA followed by Tukey's test (I-MH) (p<0.05). The %MH of Saforide® was significantly greater than that of Ag-Nano (p<0.05). Internal MH showed progressive improvement in the enamel remineralization for all cariostatic tested. In ADTs showed greater inhibition of S. mutans, E. faecalis, and E. coli by Saforide® than by Ancarie® and Ag-Nano. Ag-Nano was able to inhibit 100% microorganism growth at a lower concentration than required for the other agents. It was concluded that Ag-Nano treatment promoted remineralization of deciduous tooth enamel with initial caries-like lesion and bactericidal activity. |
