| Autor: |
Hoang Anh NT; a Institute of Chemistry , Vietnam Academy of Science and Technology , Hanoi , Vietnam.; b Graduate University of Science and Technology , Vietnam Academy of Science and Technology , Hanoi , Vietnam., Tam KT; b Graduate University of Science and Technology , Vietnam Academy of Science and Technology , Hanoi , Vietnam.; c Thai Nguyen University of Sciences , Thai Nguyen University , Thai Nguyen , Vietnam., Tuan NV; b Graduate University of Science and Technology , Vietnam Academy of Science and Technology , Hanoi , Vietnam.; d Asean College , Van Lam, Hung Yen, Vietnam., Thien DD; a Institute of Chemistry , Vietnam Academy of Science and Technology , Hanoi , Vietnam., Quan TD; a Institute of Chemistry , Vietnam Academy of Science and Technology , Hanoi , Vietnam., Tam NT; a Institute of Chemistry , Vietnam Academy of Science and Technology , Hanoi , Vietnam., Bao NC; e Department of Chemistry , Hue University , Hue , Vietnam., Do TT; f Institute of Biotechnology , Vietnam Academy of Science and Technology , Hanoi , Vietnam., Nga NT; f Institute of Biotechnology , Vietnam Academy of Science and Technology , Hanoi , Vietnam., Thuy TT; a Institute of Chemistry , Vietnam Academy of Science and Technology , Hanoi , Vietnam., Sung TV; a Institute of Chemistry , Vietnam Academy of Science and Technology , Hanoi , Vietnam., Delfino DV; g Department of Medicine , University of Perugia , Perugia, Italy. |
| Abstrakt: |
This study describes the chemical constituents of Oldenlandia pinifolia (Wall. Ex G. Don) Kuntze (synonym Hedyotis pinifolia Wall. Ex G. Don) and discusses their anti-proliferative activities. Thirteen compounds were isolated from the n-hexane, ethyl acetate and n-butanol extracts of whole plants O. pinifolia by chromatography method. Their structures were elucidated using MS and NMR analysis and compared with reported data. They are three anthraquinones, a carotenoid, two triterpenes, four iridoid glycosides and three flavonoid glycosides. Among them, 2-methyl-1,4,6-trihydroxy-anthraquinone is a new one, and three compounds were found for the first time in this genus. MTT assay resulted that the n-butanol extract and four isolated compounds inhibited the proliferation of chronic myelogenous leukaemia cells. The results from Hoechst 33343 staining and caspase 3-inducing exhibited that those four tested compounds induced apoptosis and activated caspase 3 (p < 0.05). One of them, isorhamnetin-3-O-β-rutinoside showed the most activity with IC 50 value of 394.68 ± 25.12 μM. |
