Association of TNFA (-308G/A), IFNG (+874 A/T) and IL-10 (-819 C/T) polymorphisms with protection and susceptibility to dengue in Brazilian population.

Autor:	Santos ACMD; Molecular Biology and Gene Expression Laboratory, Federal University of Alagoas, Campus Arapiraca, Av Manoel Severino Barbosa, Bom Sucesso, Arapiraca, AL, 57309-005, Brazil., de Moura EL; Molecular Biology and Gene Expression Laboratory, Federal University of Alagoas, Campus Arapiraca, Av Manoel Severino Barbosa, Bom Sucesso, Arapiraca, AL, 57309-005, Brazil., Ferreira JM; Molecular Biology and Gene Expression Laboratory, Federal University of Alagoas, Campus Arapiraca, Av Manoel Severino Barbosa, Bom Sucesso, Arapiraca, AL, 57309-005, Brazil., de Moura AWA; Molecular Biology and Gene Expression Laboratory, Federal University of Alagoas, Campus Arapiraca, Av Manoel Severino Barbosa, Bom Sucesso, Arapiraca, AL, 57309-005, Brazil., Ferreira ADS; Molecular Biology and Gene Expression Laboratory, Federal University of Alagoas, Campus Arapiraca, Av Manoel Severino Barbosa, Bom Sucesso, Arapiraca, AL, 57309-005, Brazil., Bezerra RP; Molecular Biology and Gene Expression Laboratory, Federal University of Alagoas, Campus Arapiraca, Av Manoel Severino Barbosa, Bom Sucesso, Arapiraca, AL, 57309-005, Brazil., de Siqueira Figueiredo D; Molecular Biology and Gene Expression Laboratory, Federal University of Alagoas, Campus Arapiraca, Av Manoel Severino Barbosa, Bom Sucesso, Arapiraca, AL, 57309-005, Brazil., de Farias KF; Molecular Biology and Gene Expression Laboratory, Federal University of Alagoas, Campus Arapiraca, Av Manoel Severino Barbosa, Bom Sucesso, Arapiraca, AL, 57309-005, Brazil., de Andrade TG; Molecular Biology and Gene Expression Laboratory, Federal University of Alagoas, Campus Arapiraca, Av Manoel Severino Barbosa, Bom Sucesso, Arapiraca, AL, 57309-005, Brazil; Faculty of Medicine, Federal University of Alagoas, Campus AC Simões, Tabuleiro dos Martins, Maceió, Alagoas, Brazil., de Souza Figueiredo EVM; Molecular Biology and Gene Expression Laboratory, Federal University of Alagoas, Campus Arapiraca, Av Manoel Severino Barbosa, Bom Sucesso, Arapiraca, AL, 57309-005, Brazil. Electronic address: elainevms@yahoo.com.br.
Jazyk:	angličtina
Zdroj:	Asian Pacific journal of tropical medicine [Asian Pac J Trop Med] 2017 Nov; Vol. 10 (11), pp. 1065-1071. Date of Electronic Publication: 2017 Oct 28.
DOI:	10.1016/j.apjtm.2017.10.009
Abstrakt:	Objective: To evaluate gene polymorphisms and their association with susceptibility to dengue. Methods: A retrospective case-control study was performed with 262 subjects, comprising 78 dengue fever (DF) patients, 49 dengue hemorrhagic fever (DHF) patients and 135 healthy controls. Genotypic and allelic profiles were identified using polymerase chain reaction based in real time and amplification-refractory mutation system. Results: We observed a protective association of IL-10 (-819 C/T) C allele (P = 0.028, OR = 0.56, CI = 0.34-0.91) against DHF, while the C/T (P = 0.047, OR = 2.10, CI = 1.01-4.38) and T/T (P = 0.008, OR = 3.82, CI = 1.38-10.59) genotypes were associated with DHF and DF, respectively. The dominant model TNFA -308 GA + AA (P = 0.043, OR = 0.45, CI = 0.20-1.00) genotypes were found to have protective effect against dengue infection. A protective association among the IFNG (+874 A/T) A/T genotype against DF (P = 0.02, OR = 0.46, CI = 0.24-0.89) and DHF (P = 0.034, OR = 0.43, CI = 0.19-0.95) was observed. When the studied single-nucleotide polymorphism was analyzed in combination, the combination GTA (P = 0.022, OR = 2.95, CI = 1.18-7.41) was statistically significantly associated with susceptibility to DF and the combination GCT (P = 0.035, OR = 0.28, CI = 0.08-0.90) with protection against the development of DHF. Conclusions: This research identifies the association of the IFNG (+874 A/T), TNFA (-308G/A), IL-10 (-819 C/T) genotypes as a factor for protection, susceptibility and severity to dengue. (Copyright © 2017 Hainan Medical University. Production and hosting by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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