Hepatitis B Core Antigen in Hepatocytes of Chronic Hepatitis B: Comparison between Indirect Immunofluorescence and Immunoperoxidase Method.

Autor:	Raihan R; Department of Microbiology, Faculty of Medicine, AIMST University, Bedong, Kedah, Malaysia.; Department of Virology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh., Tabassum S; Department of Microbiology, Faculty of Medicine, AIMST University, Bedong, Kedah, Malaysia., Al-Mahtab M; Department of Hepatology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh., Nessa A; Department of Virology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh., Jahan M; Department of Virology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh., Shamim Kabir CM; Department of Medicine, Shaheed Suhrawardy Medical College and Hospital, Mirpur, Dhaka, Bangladesh., Kamal M; Department of Pathology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh., Cesar Aguilar J; Department of Biomedical Research, Centre for Genetic Engineering and Biotechnology, Havana City, Cuba.
Jazyk:	angličtina
Zdroj:	Euroasian journal of hepato-gastroenterology [Euroasian J Hepatogastroenterol] 2015 Jan-Jun; Vol. 5 (1), pp. 7-10. Date of Electronic Publication: 2015 Jan 06.
DOI:	10.5005/jp-journals-10018-1120
Abstrakt:	Background: Hepatitis B virus (HBV) infection has many faces. Precore and core promoter mutants resemble inactive carrier status. The identification of hepatitis B core antigen (HBcAg) in hepatocytes may have variable clinical significance. The present study was undertaken to detect HBcAg in chronic hepatitis B (CHB) patients and to assess the efficacy of detection system by indirect immunofluorescence (IIF) and indirect immunoperoxidase (IIP). Materials and Methods: The study was done in 70 chronic HBV-infected patients. Out of 70 patients, eight (11.4%) were hepatitis B e antigen (HBeAg) positive and 62 (88.57%) were HBeAg negative. Hepatitis B core antigen was detected by indirect immunofluorescence (IIF) and indirect immunoperoxidase (IIP) methods in liver tissue. Results: All HBeAg positive patients expressed HBcAg by both IIF and IIP methods. Out of 62 patients with HBeAg-negative CHB, HBcAg was detected by IIF in 55 (88.7%) patients and by IIP in 51 (82.26%) patients. A positive relation among viral load and HBcAg detection was also found. This was more evident in the case of HBeAg negative patients and showed a positive relation with HBV DNA levels. Conclusion: Hepatitis B core antigen can be detected using the IIF from formalin fixed paraffin block preparation and also by IIP method. This seems to reflect the magnitudes of HBV replication in CHB. How to Cite This Article: Raihan R, Tabassum S, Al-Mahtab M, Nessa A, Jahan M, Kabir CMS, Kamal M, Aguilar JC. Hepatitis B Core Antigen in Hepatocytes of Chronic Hepatitis B: Comparison between Indirect Immunofluorescence and Immunoperoxidase Method. Euroasian J Hepato-Gastroenterol 2015;5(1):7-10. Competing Interests: Source of support: Nil Conflict of interest: None
Databáze:	MEDLINE
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