| Autor: |
Alvares K; Department of Pathology, Northwestern University Medical School, Chicago, Illinois 60611., Nemali MR, Reddy PG, Wang XD, Rao MS, Reddy JK |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1989 Feb 15; Vol. 158 (3), pp. 991-5. |
| DOI: |
10.1016/0006-291x(89)92820-9 |
| Abstrakt: |
Recently we reported the sequence of a cDNA clone (pUOX-1), isolated from a lambda gt11 cDNA library, which encoded for rat liver urate oxidase (EC 1.7.3.3), but this clone lacked the nucleotide sequences encoding the N-terminal region for this enzyme. Using the cDNA insert from the pUOX-1 clone as a probe, we have now isolated a full length cDNA clone, pUOX-2, from a lambda gt10 library by plaque hybridization. Nucleotide sequence analysis of the pUOX-2 clone showed that it has 1379 base pairs with an open reading frame coding for 303 amino acid residues corresponding to a molecular mass of 34,931 daltons. In addition to the open reading frame the pUOX-2 contains 439 bp of 3'-untranslated and 41 bp of 5'-untranslated sequences. The consensus polyadenylation signal AATAAA precedes a stretch of poly(A)+ residues at the 3' end. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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