Chitosan produced from Mucorales fungi using agroindustrial by-products and its efficacy to inhibit Colletotrichum species.

Autor:	Ramos Berger LR; Universidade Federal da Paraíba, Centro de Ciências da Saúde, Cidade Universitária, 58.051-900, João Pessoa, Paraíba, Brazil. Electronic address: luciaraquelramosberger@gmail.com., Montenegro Stamford TC; Universidade Federal de Pernambuco, Centro de Ciências da Saúde,Av. Prof. Moraes Rego, n° 1235, Cidade Universitária, 50.670-901, Recife, Pernambuco, Brazil., de Oliveira KÁR; Universidade Federal da Paraíba, Centro de Ciências da Saúde, Cidade Universitária, 58.051-900, João Pessoa, Paraíba, Brazil., de Miranda Pereira Pessoa A; Universidade Federal da Paraíba, Centro de Ciências da Saúde, Cidade Universitária, 58.051-900, João Pessoa, Paraíba, Brazil., de Lima MAB; Universidade Federal Rural de Pernambuco,Av. Dom Manoel de Medeiros, Dois Irmãos, 52.171-900, Recife, Pernambuco, Brazil., Estevez Pintado MM; Universidade Católica Portuguesa, Campus da Asprela, Rua Dr. António Bernardino de Almeida, 4200-072, Porto, Portugal., Saraiva Câmara MP; Universidade Federal Rural de Pernambuco,Av. Dom Manoel de Medeiros, Dois Irmãos, 52.171-900, Recife, Pernambuco, Brazil., de Oliveira Franco L; Universidade Federal Rural de Pernambuco,Av. Dom Manoel de Medeiros, Dois Irmãos, 52.171-900, Recife, Pernambuco, Brazil., Magnani M; Universidade Federal da Paraíba, Centro de Ciências da Saúde, Cidade Universitária, 58.051-900, João Pessoa, Paraíba, Brazil., de Souza EL; Universidade Federal da Paraíba, Centro de Ciências da Saúde, Cidade Universitária, 58.051-900, João Pessoa, Paraíba, Brazil.
Jazyk:	angličtina
Zdroj:	International journal of biological macromolecules [Int J Biol Macromol] 2018 Mar; Vol. 108, pp. 635-641. Date of Electronic Publication: 2017 Dec 02.
DOI:	10.1016/j.ijbiomac.2017.11.178
Abstrakt:	This study evaluated corn steep liquor (CSL) and papaya peel juice (PPJ) in mixture as substrates for the cultivation (96h, 28°C, pH 5.6, 150rpm) of Mucorales fungi for chitosan production, and determined the growth-inhibitory effect of the fungal chitosan (FuCS) obtained under optimized conditions against phytopathogenic Colletotrichum species. All Mucorales fungi tested were capable of growing in CSL-PPJ medium, showing FuCS production in the range of 5.02 (Fennelomyces heterothalicus SIS 28) - 15.63mg/g (Cunninghamella elegans SIS 41). Highest FuCS production (37.25mg/g) was achieved when C. elegans was cultivated in medium containing 9.43% CSL and 42.5% PPJ. FuCS obtained under these conditions showed a deacetylation degree of 86%, viscosity of 120cP and molecular weight of 4.08×10 4 g/mol. FuCS at 5000, 7500 and 10,000ppm inhibited the growth of all Colletotrichum species tested. FuCS also induced alterations in the morphology of C. fructicola hyphae. CSL-PPJ mixtures are suitable substrates for the cultivation of Mucorales fungi for FuCS production. Chitosan from C. elegans cultivated in CSL-PPJ medium is effective in inhibiting phytopathogenic Colletotrichum species. (Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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