The Kalanchoë genome provides insights into convergent evolution and building blocks of crassulacean acid metabolism.

Autor: Yang X; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA. yangx@ornl.gov.; The Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville, TN, 37996, USA. yangx@ornl.gov., Hu R; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Yin H; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Jenkins J; HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL, 35801, USA., Shu S; US Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA, 94598, USA., Tang H; Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China., Liu D; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Weighill DA; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.; The Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville, TN, 37996, USA., Cheol Yim W; Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, 89557, USA., Ha J; Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, 89557, USA., Heyduk K; Department of Plant Biology, University of Georgia, Athens, GA, 30602, USA., Goodstein DM; US Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA, 94598, USA., Guo HB; Department of Biochemistry & Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, 37996, USA., Moseley RC; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.; The Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville, TN, 37996, USA., Fitzek E; Department of Biological Sciences, Northern Illinois University, DeKalb, IL, 60115, USA., Jawdy S; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Zhang Z; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Xie M; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Hartwell J; Department of Plant Sciences, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK., Grimwood J; HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL, 35801, USA., Abraham PE; Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Mewalal R; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Beltrán JD; Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, UK., Boxall SF; Department of Plant Sciences, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK., Dever LV; Department of Plant Sciences, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK., Palla KJ; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.; The Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville, TN, 37996, USA., Albion R; Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, 89557, USA., Garcia T; Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, 89557, USA., Mayer JA; Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, 89557, USA., Don Lim S; Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, 89557, USA., Man Wai C; Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA., Peluso P; Pacific Biosciences, Inc., 940 Hamilton Avenue, Menlo Park, CA, 94025, USA., Van Buren R; Department of Horticulture, Michigan State University, East Lansing, MI, 48824, USA., De Paoli HC; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.; Department of Plant Sciences, University of Tennessee, Knoxville, TN, 37996, USA., Borland AM; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.; School of Natural and Environmental Science, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK., Guo H; Department of Biochemistry & Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, 37996, USA., Chen JG; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Muchero W; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Yin Y; Department of Biological Sciences, Northern Illinois University, DeKalb, IL, 60115, USA., Jacobson DA; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.; The Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville, TN, 37996, USA., Tschaplinski TJ; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Hettich RL; Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Ming R; Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.; Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA., Winter K; Smithsonian Tropical Research Institute, Apartado, Balboa, Ancón, 0843-03092, Republic of Panama., Leebens-Mack JH; Department of Plant Biology, University of Georgia, Athens, GA, 30602, USA., Smith JAC; Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, UK., Cushman JC; Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, 89557, USA., Schmutz J; HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL, 35801, USA.; US Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA, 94598, USA., Tuskan GA; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2017 Dec 01; Vol. 8 (1), pp. 1899. Date of Electronic Publication: 2017 Dec 01.
DOI: 10.1038/s41467-017-01491-7
Abstrakt: Crassulacean acid metabolism (CAM) is a water-use efficient adaptation of photosynthesis that has evolved independently many times in diverse lineages of flowering plants. We hypothesize that convergent evolution of protein sequence and temporal gene expression underpins the independent emergences of CAM from C 3 photosynthesis. To test this hypothesis, we generate a de novo genome assembly and genome-wide transcript expression data for Kalanchoë fedtschenkoi, an obligate CAM species within the core eudicots with a relatively small genome (~260 Mb). Our comparative analyses identify signatures of convergence in protein sequence and re-scheduling of diel transcript expression of genes involved in nocturnal CO 2 fixation, stomatal movement, heat tolerance, circadian clock, and carbohydrate metabolism in K. fedtschenkoi and other CAM species in comparison with non-CAM species. These findings provide new insights into molecular convergence and building blocks of CAM and will facilitate CAM-into-C 3 photosynthesis engineering to enhance water-use efficiency in crops.
Databáze: MEDLINE