| Autor: |
Lin C; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Fesi BD; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Marquis M; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Bosak NP; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Lysenko A; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Koshnevisan MA; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Duke FF; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Theodorides ML; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Nelson TM; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., McDaniel AH; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Avigdor M; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Arayata CJ; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Shaw L; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Bachmanov AA; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America., Reed DR; Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America. |
| Abstrakt: |
An average mouse in midlife weighs between 25 and 30 g, with about a gram of tissue in the largest adipose depot (gonadal), and the weight of this depot differs between inbred strains. Specifically, C57BL/6ByJ mice have heavier gonadal depots on average than do 129P3/J mice. To understand the genetic contributions to this trait, we mapped several quantitative trait loci (QTLs) for gonadal depot weight in an F2 intercross population. Our goal here was to fine-map one of these QTLs, Adip20 (formerly Adip5), on mouse chromosome 9. To that end, we analyzed the weight of the gonadal adipose depot from newly created congenic strains. Results from the sequential comparison method indicated at least four rather than one QTL; two of the QTLs were less than 0.5 Mb apart, with opposing directions of allelic effect. Different types of evidence (missense and regulatory genetic variation, human adiposity/body mass index orthologues, and differential gene expression) implicated numerous candidate genes from the four QTL regions. These results highlight the value of mouse congenic strains and the value of this sequential method to dissect challenging genetic architecture. |
