[Removal of lipopolysaccharides from anti-complementary crude polysaccharides of Houttuynia Herba].

Autor:	Zhang JJ; School of Pharmacy, Fudan University, Shanghai 201203, China., Zhang QL; School of Pharmacy, Fudan University, Shanghai 201203, China., Lu Y; School of Pharmacy, Fudan University, Shanghai 201203, China., Chen DF; School of Pharmacy, Fudan University, Shanghai 201203, China.
Jazyk:	čínština
Zdroj:	Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2017 Sep; Vol. 42 (17), pp. 3398-3402.
DOI:	10.19540/j.cnki.cjcmm.20170728.011
Abstrakt:	An Affi-Prep Polymyxin column was combined with a Phenyl Sepharose column and a Sephacryl S-300 column, respectively, to remove the lipopolysaccharides(LPS) in the anti-complementary crude polysaccharides of Houttuynia Herba. The contents of LPS in the polysaccharides were determined by chromogenic tachypleus amebocyte lysate(TAL)method during the procedure of purifying. The anti-complementary activities of the polysaccharides were also compared before and after the removal of LPS. Less remanent LPS was detected after purified using Penyl Sepharose combined with polymyxin column, with the clearance rate of 42.85%. All the columns had no effect on the anti-complementary activity of the polysaccharides. Penyl Sepharose combined with polymyxin column would be sound for LPS removal of the anti-complementary polysaccharides without reducing their bioactivity. Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose. (Copyright© by the Chinese Pharmaceutical Association.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu