| Autor: |
Mizinga KM; Elanco Animal Health, 2500 Innovation Way, Greenfield, IN 46140., Burnett TJ; Elanco Animal Health, 2500 Innovation Way, Greenfield, IN 46140., Brunelle SL; Brunelle Biotech Consulting, 6620 NW Burgundy Dr., Corvallis, OR 97330., Wallace MA; MPI Research, Inc., 3058 Research Dr., State College, PA 16801., Coleman MR; Mark R. Coleman, PhD, Consulting, LLC, 126 Yorkshire Blvd. E, Indianapolis, IN 46229. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of AOAC International [J AOAC Int] 2018 May 01; Vol. 101 (3), pp. 891-896. Date of Electronic Publication: 2017 Dec 01. |
| DOI: |
10.5740/jaoacint.17-0203 |
| Abstrakt: |
The U.S. Department of Agriculture, Food Safety Inspection Service regulatory method for monensin, Chemistry Laboratory Guidebook CLG-MON, is a semiquantitative bioautographic method adopted in 1991. Official Method of AnalysisSM (OMA) 2011.24, a modern quantitative and confirmatory LC-tandem MS method, uses no chlorinated solvents and has several advantages, including ease of use, ready availability of reagents and materials, shorter run-time, and higher throughput than CLG-MON. Therefore, a bridging study was conducted to support the replacement of method CLG-MON with OMA 2011.24 for regulatory use. Using fortified bovine tissue samples, CLG-MON yielded accuracies of 80-120% in 44 of the 56 samples tested (one sample had no result, six samples had accuracies of >120%, and five samples had accuracies of 40-160%), but the semiquantitative nature of CLG-MON prevented assessment of precision, whereas OMA 2011.24 had accuracies of 88-110% and RSDr of 0.00-15.6%. Incurred residue results corroborated these results, demonstrating improved accuracy (83.3-114%) and good precision (RSDr of 2.6-20.5%) for OMA 2011.24 compared with CLG-MON (accuracy generally within 80-150%, with exceptions). Furthermore, χ2 analysis revealed no statistically significant difference between the two methods. Thus, the microbiological activity of monensin correlated with the determination of monensin A in bovine tissues, and OMA 2011.24 provided improved accuracy and precision over CLG-MON. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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