The Microbead Occlusion Model of Ocular Hypertension in Mice.

Autor: Calkins DJ; The Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, TN, 37232, USA. david.j.calkins@vanderbilt.edu.; Department of Ophthalmology and Visual Sciences, Vanderbilt University School of Medicine, Nashville, TN, USA. david.j.calkins@vanderbilt.edu., Lambert WS; The Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, TN, 37232, USA., Formichella CR; The Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, TN, 37232, USA., McLaughlin WM; The Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, TN, 37232, USA., Sappington RM; The Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, TN, 37232, USA. rebecca.m.sappington@vanderbilt.edu.; Department of Ophthalmology and Visual Sciences, Vanderbilt University School of Medicine, Nashville, TN, USA. rebecca.m.sappington@vanderbilt.edu.; Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN, USA. rebecca.m.sappington@vanderbilt.edu.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1695, pp. 23-39.
DOI: 10.1007/978-1-4939-7407-8_3
Abstrakt: Glaucoma is a common optic neuropathy that leads to vision loss through the degeneration of retinal ganglion cells (RGCs) and their axons. RGC degeneration in glaucoma is associated with sensitivity to intraocular pressure (IOP) and elevated IOP (also known as ocular hypertension) is the primary modifiable risk factor. Ocular hypertension is the primary characteristic of rodent models for glaucoma research. Intracameral injection of microbeads has evolved as a preferred method of IOP elevation in rodents, particularly in mice. Here, we outline the protocol and method for the Microbead Occlusion Model in mice. We highlight the importance of anesthesia choice and the utilization of glass micropipettes in combination with a micromanipulator and microsyringe pump for the successful execution of the model.
Databáze: MEDLINE
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