A colorimetric competitive displacement assay for the evaluation of catalytic peptides.

Autor: Gest AMM; Department of Chemistry, Macalester College, 1600 Grand Ave., Saint Paul, MN 55105, USA. lwitus@macalester.edu., Aguiluz EM, Mays MT, Liu X, Neidhart EK, Witus LS
Jazyk: angličtina
Zdroj: Organic & biomolecular chemistry [Org Biomol Chem] 2017 Dec 13; Vol. 15 (48), pp. 10160-10163.
DOI: 10.1039/c7ob02032e
Abstrakt: An indicator displacement assay has been adapted to detect the diol products of the aldol reaction between 4-nitrobenzaldehyde and hydroxyacetone in crude reaction mixtures. This provides a rapid colorimetric method of detecting product formation and thus evaluating potential catalysts, which is demonstrated using multiple catalytic peptides.
Databáze: MEDLINE