| Autor: |
Ruh AC; Biological and Health Science, Multidisciplinary Laboratory, State University of Ponta Grossa (UEPG), Av. Gal. Carlos Cavalcanti, 4748, Ponta Grossa, PR, 84030-900, Brazil., Frigo L; Laser in Dentistry Program, Cruzeiro do Sul University (UNICSUL), Av. Dr. Ussiel Cirilo 225, São Miguel Paulista, SP, 08060-070, Brazil.; Rua Galvão Bueno 868, Liberdade, SP, 01506-000, Brazil., Cavalcanti MFXB; Laser in Dentistry Program, Cruzeiro do Sul University (UNICSUL), Av. Dr. Ussiel Cirilo 225, São Miguel Paulista, SP, 08060-070, Brazil. mxistocavalcanti@gmail.com.; Rua Galvão Bueno 868, Liberdade, SP, 01506-000, Brazil. mxistocavalcanti@gmail.com.; Faculté de Médecine, UMR 7365 CNRS-Université de Lorraine, 9, avenue de la Forêt de Haye, 54500, Vandoeuvre-lés-Nancy, France. mxistocavalcanti@gmail.com., Svidnicki P; Departamento de Biologia Estrutural, Molecular e Genética, State University of Ponta Grossa (UEPG), Av. Carlos Cavalcanti, 4748, Ponta Grossa, PR, 84030-900, Brazil., Vicari VN; Departamento de Biologia Estrutural, Molecular e Genética, State University of Ponta Grossa (UEPG), Av. Carlos Cavalcanti, 4748, Ponta Grossa, PR, 84030-900, Brazil., Lopes-Martins RAB; Center for Research and Innovation in Laser, Nove de Julho University (UNINOVE), Rua Vergueiro 235, São Paulo, SP, 01504-001, Brazil.; Technologycal Innovation Center, Mogi das Cruzes University, Av. Candido Almeida de Xavier e Souza, 200, Mogi das Cruzes, SP, 08780-911, Brazil., Leal Junior ECP; Center for Research and Innovation in Laser, Nove de Julho University (UNINOVE), Rua Vergueiro 235, São Paulo, SP, 01504-001, Brazil., De Isla N; Faculté de Médecine, UMR 7365 CNRS-Université de Lorraine, 9, avenue de la Forêt de Haye, 54500, Vandoeuvre-lés-Nancy, France., Diomede F; Department of Medical, Oral and Biotechnological Sciences, 'G. d'Annunzio' University, Chieti-Pescara, 66100, Chieti, Italy., Trubiani O; Department of Medical, Oral and Biotechnological Sciences, 'G. d'Annunzio' University, Chieti-Pescara, 66100, Chieti, Italy., Favero GM; Biological and Health Science, Multidisciplinary Laboratory, State University of Ponta Grossa (UEPG), Av. Gal. Carlos Cavalcanti, 4748, Ponta Grossa, PR, 84030-900, Brazil. |
| Abstrakt: |
Pressure ulcers (PU) are wounds located mainly on bone surfaces where the tissue under pressure suffers ischemia leading to cellular lesion and necrosis , its causes and the healing process depend on several factors. The aim of this study was evaluating the gene expression of inflammatory/reparative factors: IL6, TNF, VEGF, and TGF, which take part in the tissue healing process under effects of low-level laser therapy (LLLT). In order to perform lesion area analysis, PUs were photographed and computer analyzed. Biochemical analysis was performed sa.mpling ulcer border tissue obtained through biopsy before and after laser therapy and quantitative real-time PCR (qRT-PCR) analysis. The study comprised eight individuals, mean age sixty-two years old, and sacroiliac and calcaneous PU, classified as degree III and IV according to the National Pressure Ulcer Advisory Panel (NPUAP). PUs were irradiated with low-level laser (InGaAIP, 100 mW, 660 nm), energy density 2 J/cm 2 , once a day, with intervals of 24 h, totaling 12 applications. The lesion area analysis revealed averaged improvement of the granulation tissue size up to 50% from pre- to post-treatment. qRT-PCR analysis revealed that IL6 values were not significantly different before and after treatment, TNF gene expression was reduced, and VEFG and TGF-β gene expression increased after treatment. After LLLT, wounds presented improvement in gross appearance, with increase in factors VEFG and TGF-β, and reduction of TNF; despite our promising results, they have to be analyzed carefully as this study did not have a control group. |
Full text from SpringerLink