On the contributions of photorespiration and compartmentation to the contrasting intramolecular 2 H profiles of C 3 and C 4 plant sugars.

Autor: Zhou Y; School of Chemistry & Chemical Engineering, Shaanxi University of Science & Technology, Xi'an, China; Institute for Landscape Biogeochemistry, ZALF, Germany; Leibniz Institute for Freshwater Ecology & Inland Fisheries, Germany. Electronic address: youping.zhou@sust.edu.cn., Zhang B; School of Chemistry & Chemical Engineering, Shaanxi University of Science & Technology, Xi'an, China., Stuart-Williams H; Research School of Biology, Australian National University, Australia., Grice K; WA-Organic and Isotope Geochemistry Centre, Department of Chemistry, Curtin University, Australia., Hocart CH; School of Chemistry & Chemical Engineering, Shaanxi University of Science & Technology, Xi'an, China; Research School of Biology, Australian National University, Australia., Gessler A; Institute for Landscape Biogeochemistry, ZALF, Germany; Swiss Federal Institute for Forest, Snow and Landscape Research, Birmensdorf, Switzerland., Kayler ZE; Institute for Landscape Biogeochemistry, ZALF, Germany; USDA Forest Service, Northern Research Station, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA., Farquhar GD; Research School of Biology, Australian National University, Australia.
Jazyk: angličtina
Zdroj: Phytochemistry [Phytochemistry] 2018 Jan; Vol. 145, pp. 197-206. Date of Electronic Publication: 2017 Nov 28.
DOI: 10.1016/j.phytochem.2017.11.004
Abstrakt: Compartmentation of C 4 photosynthetic biochemistry into bundle sheath (BS) and mesophyll (M) cells, and photorespiration in C 3 plants is predicted to have hydrogen isotopic consequences for metabolites at both molecular and site-specific levels. Molecular-level evidence was recently reported (Zhou et al., 2016), but evidence at the site-specific level is still lacking. We propose that such evidence exists in the contrasting 2 H distribution profiles of glucose samples from naturally grown C 3 , C 4 and CAM plants: photorespiration contributes to the relative 2 H enrichment in H 5 and relative 2 H depletion in H 1 & H 6 (the average of the two pro-chiral Hs and in particular H 6, pro-R ) in C 3 glucose, while 2 H-enriched C 3 mesophyll cellular (chloroplastic) water most likely contributes to the enrichment at H 4 ; export of (transferable hydrogen atoms of) NADPH from C 4 mesophyll cells to bundle sheath cells (via the malate shuttle) and incorporation of 2 H-relatively unenriched BS cellular water contribute to the relative depletion of H 4 & H 5 respectively; shuttling of triose-phosphates (PGA: phosphoglycerate dand DHAP: dihydroacetone phosphate) between C 4 bundle sheath and mesophyll cells contributes to the relative enrichment in H 1 & H 6 (in particular H 6, pro-R ) in C 4 glucose.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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