Design, optimisation and preliminary validation of a human specific loop-mediated amplification assay for the rapid detection of human DNA at forensic crime scenes.

Autor: Hird HJ; Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, United Kingdom. Electronic address: hez.hird@fera.gsi.gov.uk., Brown MK; School of Science and Engineering, Teesside University, Borough Road, Middlesbrough TS1 3BA, United Kingdom.
Jazyk: angličtina
Zdroj: Science & justice : journal of the Forensic Science Society [Sci Justice] 2017 Nov; Vol. 57 (6), pp. 409-414. Date of Electronic Publication: 2017 Aug 03.
DOI: 10.1016/j.scijus.2017.08.001
Abstrakt: The identification of samples at a crime scene which require forensic DNA typing has been the focus of recent research interest. We propose a simple, but sensitive analysis system which can be deployed at a crime scene to identify crime scene stains as human or non-human. The proposed system uses the isothermal amplification of DNA in a rapid assay format, which returns results in as little as 30min from sampling. The assay system runs on the Genie II device, a proven in-field detection system which could be deployed at a crime scene. The results presented here demonstrate that the system was sufficiently specific and sensitive and was able to detect the presence of human blood, semen and saliva on mock forensic samples.
(Copyright © 2017. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: