Regulation of UGT1A expression by miR-298 in human livers from the Han Chinese population and in human cell lines.

Autor:	Wang P; Department of Pharmacology, School of Basic Medicine, Zhengzhou University, Zhengzhou, China., Nie YL; Department of Pharmacology, School of Basic Medicine, Zhengzhou University, Zhengzhou, China., Wang SJ; Department of Pharmacology, School of Pharmacy, Zhengzhou University, Zhengzhou, China., Yang LL; Department of Pharmacology, School of Basic Medicine, Zhengzhou University, Zhengzhou, China., Yang WH; Department of Pharmacology, School of Basic Medicine, Zhengzhou University, Zhengzhou, China., Li JF; Department of Pharmacology, School of Basic Medicine, Zhengzhou University, Zhengzhou, China., Li XT; Department of Pharmacology, School of Pharmacy, Zhengzhou University, Zhengzhou, China., Zhang LR; Department of Pharmacology, School of Basic Medicine, Zhengzhou University, Zhengzhou, China.
Jazyk:	angličtina
Zdroj:	Epigenomics [Epigenomics] 2018 Jan; Vol. 10 (1), pp. 43-57. Date of Electronic Publication: 2017 Nov 27.
DOI:	10.2217/epi-2017-0068
Abstrakt:	Aim: This study aimed to investigate the role of miRNAs in UGT1A regulation. Materials & Methods: Based on bioinformatic prediction results, luciferase reporter assay and cell-transfection experiments were performed to study effects of miR-298 on UGT1A expression. Correlation study was conducted in human livers. Results: miR-298 overexpression reduced mRNA level of UGT1A1 and UGT1A4 in HepG2 and LS174T cells, and that of UGT1A3 and UGT1A9 in LS174T cells. miR-298 repression increased mRNA level of UGT1A4 in HepG2 and LS174T cells, and that of UGT1A1 and UGT1A3 in LS174T cells. Inverse correlations between miR-298, as well as miR-491-3p, and UGT1A3 and 1A4 mRNA levels were observed in livers. Conclusion: The study demonstrates that miR-298 and miR-491-3p downregulates UGT1A expression.
Databáze:	MEDLINE
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