Evaluation of photosensitizer penetration into sound and decayed dentin: A photoacoustic spectroscopy study.

Autor: Occhi-Alexandre IGP; Dentistry Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, CEP 87020-900, Brazil., Baesso ML; Physics Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, CEP 87020-900, Brazil., Sato F; Physics Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, CEP 87020-900, Brazil., de Castro-Hoshino LV; Physics Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, CEP 87020-900, Brazil., Rosalen PL; Physiological Sciences Department, School of Dentistry of Piracicaba, University of Campinas, Avenida Limeira, 901 - Bairro Areião, Piracicaba, SP, CEP 13414-903, Brazil., Terada RSS; Dentistry Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, CEP 87020-900, Brazil., Neto AM; Physics Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, CEP 87020-900, Brazil., Fujimaki M; Dentistry Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, CEP 87020-900, Brazil. Electronic address: mfujimaki@uem.br.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2018 Mar; Vol. 21, pp. 108-114. Date of Electronic Publication: 2017 Nov 21.
DOI: 10.1016/j.pdpdt.2017.11.008
Abstrakt: Background: Photodynamic therapy (PDT) may have topical indications. In those cases it is important for a topical photosensitizer to penetrate into the tissue to which it has been applied. This study aimed to compare the penetration of two different concentrations of erythrosine into intact and in vitro decayed dentin samples.
Methods: This in vitro study evaluated erythrosine (0.3 and 5%) penetration into sound (intact) and decayed dentin. A total of 11 dentin discs were prepared and divided into two equal halves, in order to keep one half sound while the other half was submitted to sterilization and an in vitro demineralization model for 5 days. Before erythrosine application, the organic and inorganic composition of all samples was evaluated by Fourier Transform Raman spectroscopy, and after erythrosine application for 30 min, the penetration depth was determined by Photoacoustic spectroscopy technique.
Results: The results indicated that 0.3% erythrosine showed a higher penetration depth into sound dentin (p = 0.002); and 5% erythrosine higher penetration into decayed dentin (p < 0.001). However considering clinical parameters, no statistically significant difference was found between any of the conditions tested.
Conclusions: Erythrosine demonstrated ability to penetrate into dentin, irrespective of sound or decayed condition. Photoacoustic spectroscopy can be considered a method for estimating the penetration into hard tissues, and in conjunction with Raman spectroscopy, these are effective methods for evaluating the spectral response of dentin. Considering that erythrosine is capable of penetrating into decayed dentin, clinical trials are needed to test the effectiveness of this photosensitizer in Photodynamic therapy and Antimicrobial Photodynamic therapy.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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