Intestinal Ralstonia pickettii augments glucose intolerance in obesity.

Autor: Udayappan SD; Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands., Kovatcheva-Datchary P; Wallenberg Laboratory, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden., Bakker GJ; Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands., Havik SR; Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands., Herrema H; Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands., Cani PD; Université catholique de Louvain, WELBIO (Walloon Excellence in Life sciences and BIOtechnology), Louvain Drug Research Institute, Brussels, Belgium., Bouter KE; Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands., Belzer C; Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands., Witjes JJ; Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands., Vrieze A; Department of Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands., de Sonnaville ESV; Department of Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands., Chaplin A; Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands., van Raalte DH; Diabetes Center, Department of Internal medicine, VU University Medical Center, Amsterdam, The Netherlands.; ICAR, VU University Medical Center, Amsterdam, The Netherlands., Aalvink S; Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands., Dallinga-Thie GM; Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands., Heilig HGHJ; Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands., Bergström G; Wallenberg Laboratory, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden., van der Meij S; Department of Surgery, Flevo Hospital, Almere, The Netherlands., van Wagensveld BA; Department of Surgery, Sint Lucas Andreas Hospital, Amsterdam, The Netherlands., Hoekstra JBL; Department of Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands., Holleman F; Department of Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands., Stroes ESG; Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands., Groen AK; Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands.; Department of Pediatrics, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands., Bäckhed F; Wallenberg Laboratory, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden.; Novo Nordisk Foundation Center for Basic Metabolic Research, Section for Metabolic Receptology and Enteroendocrinology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark., de Vos WM; Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.; RPU Immunobiology, University of Helsinki, Helsinki, Finland., Nieuwdorp M; Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands.; Wallenberg Laboratory, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden.; Department of Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands.; Diabetes Center, Department of Internal medicine, VU University Medical Center, Amsterdam, The Netherlands.; ICAR, VU University Medical Center, Amsterdam, The Netherlands.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2017 Nov 22; Vol. 12 (11), pp. e0181693. Date of Electronic Publication: 2017 Nov 22 (Print Publication: 2017).
DOI: 10.1371/journal.pone.0181693
Abstrakt: An altered intestinal microbiota composition has been implicated in the pathogenesis of metabolic disease including obesity and type 2 diabetes mellitus (T2DM). Low grade inflammation, potentially initiated by the intestinal microbiota, has been suggested to be a driving force in the development of insulin resistance in obesity. Here, we report that bacterial DNA is present in mesenteric adipose tissue of obese but otherwise healthy human subjects. Pyrosequencing of bacterial 16S rRNA genes revealed that DNA from the Gram-negative species Ralstonia was most prevalent. Interestingly, fecal abundance of Ralstonia pickettii was increased in obese subjects with pre-diabetes and T2DM. To assess if R. pickettii was causally involved in development of obesity and T2DM, we performed a proof-of-concept study in diet-induced obese (DIO) mice. Compared to vehicle-treated control mice, R. pickettii-treated DIO mice had reduced glucose tolerance. In addition, circulating levels of endotoxin were increased in R. pickettii-treated mice. In conclusion, this study suggests that intestinal Ralstonia is increased in obese human subjects with T2DM and reciprocally worsens glucose tolerance in DIO mice.
Databáze: MEDLINE