| Autor: |
Stenzel T, Dziewulska D, Śmiałek M, Tykałowski B, Kowalczyk J, Koncicki A |
| Jazyk: |
angličtina |
| Zdroj: |
Polish journal of veterinary sciences [Pol J Vet Sci] 2017 Sep 26; Vol. 20 (3), pp. 599-601. |
| DOI: |
10.1515/pjvs-2017-0073 |
| Abstrakt: |
The aim of this study was to develop rapid molecular assays for differentiating vaccine strains Ma5 and 4/91 of the infectious bronchitis virus (IBV). Specific primers and probes for S1 and N genes were designed based on the nucleotide sequences of both vaccine strains. Cross-reactivity was not observed. Assay sensitivity was 2.373 × 103 copies of the Ma5 strain, and 3.852 x 103 copies of the 4/91 strain. Samples belonging to a known genotype demonstrated that the designed assays supported rapid and sensitive detection of Ma5 and 4/91 vaccine strains of IBV. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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