| Autor: |
Gilliland WM Jr, Mellors JS; 908 Devices, Inc. , Boston, Massachusetts 02210, United States., Ramsey JM |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical chemistry [Anal Chem] 2017 Dec 19; Vol. 89 (24), pp. 13320-13325. Date of Electronic Publication: 2017 Dec 05. |
| DOI: |
10.1021/acs.analchem.7b03484 |
| Abstrakt: |
A microchip electrospray ionization source was coupled with high pressure mass spectrometry (HPMS). A continuous atmospheric inlet consisting of a stainless steel capillary and DC ion optics was designed to conduct ions into the mass spectrometer. Infusions of amino acids and peptides were performed and detected with a miniature cylindrical ion trap (mini-CIT)-based mass spectrometer operated at ≥1 Torr with air as the buffer gas. Detection of glycine and thymopentin (separately) demonstrated the mass range of the mini-CIT detector could span from m/z 75 to 681. A microchip capillary electrophoresis (CE) separation with mini-CIT detection was performed, and the results were compared with detection using a commercial instrument (Waters Synapt G2). Comparable separation efficiencies were observed with both mass spectrometers as detectors, with about 6 times better signal-to-noise observed on the Synapt G2. Comparison of mass spectra in the two systems reveals similar features observed, but with wider peak widths in the mini-CIT than on the Synapt G2 as expected due to high-pressure operation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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