Bioorthogonal fluorescent labels: a review on combined forces.

Autor: Cserép GB; Address: Chemical Biology Research Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute of Organic Chemistry. H-1117 Magyar tudósok krt. 2, Budapest, Hungary., Herner A, Kele P
Jazyk: angličtina
Zdroj: Methods and applications in fluorescence [Methods Appl Fluoresc] 2015 Sep 08; Vol. 3 (4), pp. 042001. Date of Electronic Publication: 2015 Sep 08.
DOI: 10.1088/2050-6120/3/4/042001
Abstrakt: This review ventures to summarize the latest developments in bioorthogonal fluorescent imaging labels with a special focus on bioimaging applications. We briefly summarize the most preferred means of bioorthogonal tagging schemes for the labeling of specific biomolecular structures. The review is structured by the type of the fluorescent labels that can address the problems that most commonly compromise fluorescent imaging techniques, i.e. the autofluorescence of biomolecules, the background fluorescence of unreacted reagents, and photobleaching. Thus, we present (i) far-red/near-infra-red emitting dyes, (ii) fluorogenic scaffolds, and (iii) nanoparticle-based signaling platforms.
Databáze: MEDLINE