Cytotoxic, Antimitotic, and Antiproliferation Studies on Rasam : A South Indian Traditional Functional Food.

Autor: Devarajan A; Department of Pharmaceutics, Hillside College of Pharmacy and Research Centre, Bengaluru, Karnataka, India.; Department of Pharmacy, Centre for Research and Development, PRIST University, Thanjavur, Tamil Nadu, India., Mohan Maruga Raja MK; Department of Pharmacy, Centre for Research and Development, PRIST University, Thanjavur, Tamil Nadu, India.
Jazyk: angličtina
Zdroj: Pharmacognosy magazine [Pharmacogn Mag] 2017 Oct; Vol. 13 (Suppl 3), pp. S452-S457. Date of Electronic Publication: 2017 Oct 11.
DOI: 10.4103/pm.pm_138_17
Abstrakt: Background: Rasam is a traditional South Indian food, prepared using tamarind juice as a base, with a variety of spices. Rasam , with all its ingredients medicinally claimed for various ailments, is a functional food. Systematic consumption of traditional functional food provides an excellent preventive measure to ward off many diseases.
Objective: To study rasam for cytotoxic, antimitotic, and antiproliferation potential beyond its culinary and nutritional effect.
Materials and Methods: Brine shrimp lethality assay, onion root tip inhibition assay, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in Calu-6, HeLa, MCF-7 cell lines for four stage-wise samples in the preparation of rasam (RS1, RS2, RS3, and RS4) were studied.
Results: RS4, the end product of rasam showed high lethality with an LC 50 value of 38.7 μL/mL. It showed maximum antimitotic activity in a dose-dependent manner compared to other samples with an IC 50 value of 189.86 μL/mL. RS4 also showed an IC 50 value of 350.22 and 410.15 μL/mL in MCF-7 and Calu-6 cell lines, respectively.
Conclusion: From this study, we suggest that rasam is a classic example of traditional functional food and it can treat breast and lung cancer on chronic use.
Summary: Rasam , a South Indian traditional functional food, showed high lethality (LC 50 = 38.7 mL/mL) against brine shrimps Rasam also showed potential antimitotic activity (IC 50 = 189.86 mL/mL) by inhibiting the onion root tips Rasam showed an IC 50 value of 350.22 and 410.15 mL/mL against MCF-7 and Calu-6 cell lines respectively Rasam , when consumed on daily dietary basis, can treat breast and lung cancer. Abbreviations used: SS 316: Stainless Steel 316 grade; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMEM: Dulbecco's modified Eagle medium; FBS: Fetal bovine serum media; TPVG: Trypsin phosphate versene glucose; EDTA: Ethylene diamine tetra acetic acid; PBS: Phosphate buffered saline; DMSO: Dimethyl sulfoxide.
Competing Interests: There are no conflicts of interest.
Databáze: MEDLINE
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