Cone myoid elongation involves unidirectional microtubule movement mediated by dynein-1.

Autor:	Lewis TR; Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226., Zareba M; Department of Ophthalmology, Medical College of Wisconsin, Milwaukee, WI 53226., Link BA; Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226., Besharse JC; Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226 jbeshars@mcw.edu.; Department of Ophthalmology, Medical College of Wisconsin, Milwaukee, WI 53226.
Jazyk:	angličtina
Zdroj:	Molecular biology of the cell [Mol Biol Cell] 2018 Jan 15; Vol. 29 (2), pp. 180-190. Date of Electronic Publication: 2017 Nov 15.
DOI:	10.1091/mbc.E17-08-0525
Abstrakt:	Teleosts and amphibians exhibit retinomotor movements, morphological changes in photoreceptors regulated by light and circadian rhythms. Cone myoid elongation occurs during dark adaptation, leading to the positioning of the cone outer segment closer to the retinal pigment epithelium. Although it has been shown that microtubules are essential for cone myoid elongation, the underlying mechanism has not been established. In this work, we generated a transgenic line of zebrafish expressing a photoconvertible form of α-tubulin (tdEOS-tubulin) specifically in cone photoreceptors. Using superresolution structured illumination microscopy in conjunction with both pharmacological and genetic manipulation, we show that cytoplasmic dynein-1, which localizes to the junction between the ellipsoid and myoid, functions to shuttle microtubules from the ellipsoid into the myoid during the course of myoid elongation. We propose a novel model by which stationary complexes of cytoplasmic dynein-1 are responsible for the shuttling of microtubules between the ellipsoid and myoid is the underlying force for the morphological change of myoid elongation. (© 2018 Lewis et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).)
Databáze:	MEDLINE
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