Antineoplastic Effects of NF-κB Inhibition by DHMEQ (Dehydroxymethylepoxyquinomicin) Alone and in Co-treatment with Radio-and Chemotherapy in Medulloblastoma Cell Lines.

Autor:	Ramos PMM; Department of Genetics, Ribeirao Preto Medical School, University of Sao Paulo, Brazil., Pezuk JA; Department of Genetics, Ribeirao Preto Medical School, University of Sao Paulo, Brazil.; Universidade Anhaguera de Sao Paulo, UNIAN-SP, Brazil., Castro-Gamero AM; Department of Genetics, Ribeirao Preto Medical School, University of Sao Paulo, Brazil.; Institute of Natural Science, Federal University of Alfenas, Brazil., Oliveira HF; Department of Clinical Medical, Ribeirao Preto Medical School, University of Sao Paulo, Brazil., Scrideli CA; Department of Pediatric, Ribeirao Preto Medical School, University of Sao Paulo, Brazil., Umezawa K; Department of Molecular Target Medicine Screening, Aichi Medical University. Nagakute, Aichi, Japan., Tone LG; Department of Genetics, Ribeirao Preto Medical School, University of Sao Paulo, Brazil.; Department of Pediatric, Ribeirao Preto Medical School, University of Sao Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Anti-cancer agents in medicinal chemistry [Anticancer Agents Med Chem] 2018; Vol. 18 (4), pp. 541-549.
DOI:	10.2174/1871520617666171113151335
Abstrakt:	Background: NF-κB is a transcription factor involved in the transcriptional regulation of a large number of genes related to tumorigenesis in several cancer cell types, and its inhibition has been related to anticancer effect. DHMEQ (Dehydroxymethylepoxyquinomicin) is a compound that blocks the translocation of NF-κB from the cytoplasm to the nucleus, thus inhibiting its activity as a transcriptional activator. Several studies have shown the antineoplastic effects of DHMEQ in numerous tumor types, however, there are no surveys that tested their effects in MB. Objectives: The aim of the present study was to evaluate the effects of DHMEQ as NF-κB inhibitor in pediatric MB cell lines. Method: We used the UW402, UW473 and ONS-76 medulloblastoma (MB) cell lines to verify the effect of DHMEQ on proliferation, clonogenic capacity, apoptosis, cell invasion and migration, and evaluated the effect of the combination with other drugs and the potential as a radiosensitizator. Results: A significant decrease in the cell growth, a strong inhibition of the clonogenic capacity, migration and cell invasion was observed after NF-κB inhibition in the three MB cell lines. Conversely, increased level of apoptosis rates were demonstrated. Additionally, treatments with DHMEQ combined with other chemotherapeutic agents were synergic in most points, and a strong radiosensitization by this compound was observed in the three MB cell lines. Conclusion: DHMEQ has potential antitumor effect on MB cells, and it may be considered a new therapeutic agent to improve treatment approaches in MB. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.)
Databáze:	MEDLINE
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