Aspidosperma pyrifolium Has Anti-Inflammatory Properties: An Experimental Study in Mice with Peritonitis Induced by Tityus serrulatus Venom or Carrageenan.

Autor:	Souza Lima MCJ; Laboratory of Technology and Pharmaceutical Biotechnology (Tecbiofar), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Rua General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil. mairalima4@hotmail.com., Oliveira Bitencourt MA; Laboratory of Technology and Pharmaceutical Biotechnology (Tecbiofar), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Rua General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil. mariana.bitencourt@unp.br., Furtado AA; Laboratory of Technology and Pharmaceutical Biotechnology (Tecbiofar), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Rua General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil. allannyfurtado@hotmail.com., Torres-Rêgo M; Laboratory of Technology and Pharmaceutical Biotechnology (Tecbiofar), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Rua General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil. manoelatorres_rn@hotmail.com., Siqueira EMDS; Laboratory of Pharmacognosy (PNBio), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Rua General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil. siqueira_emerson@hotmail.com., Oliveira RM; Laboratory of Biotechnology of Natural Biopolymers, Department of Biochemistry, Bioscience Center, Campus Universitário, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho, 3000, Lagoa Nova 59072-970, Natal, Brazil. rmo_85@hotmail.com., Oliveira Rocha HA; Laboratory of Biotechnology of Natural Biopolymers, Department of Biochemistry, Bioscience Center, Campus Universitário, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho, 3000, Lagoa Nova 59072-970, Natal, Brazil. hugo-alexandre@uol.com.br., Ferreira Rocha KB; Laboratory of Pathology, Departament of Pathology, Federal University of Rio Grande do Norte, Rua General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil. keyla.rocha@uol.com.br., Silva-Júnior AAD; Laboratory of Technology and Pharmaceutical Biotechnology (Tecbiofar), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Rua General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil. arnobiosilva@gmail.com., Zucolotto SM; Laboratory of Pharmacognosy (PNBio), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Rua General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil. szucolotto@hotmail.com., Fernandes-Pedrosa MF; Laboratory of Technology and Pharmaceutical Biotechnology (Tecbiofar), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Rua General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil. mpedrosa@ufrnet.br.
Jazyk:	angličtina
Zdroj:	International journal of molecular sciences [Int J Mol Sci] 2017 Nov 11; Vol. 18 (11). Date of Electronic Publication: 2017 Nov 11.
DOI:	10.3390/ijms18112248
Abstrakt:	Scorpions of the genus Tityus are responsible for the majority of envenomation in Brazil, the Tityus serrulatus species being the most common and dangerous in South America. In this approach, we have investigated the ability of the aqueous extract from the leaves of Aspidosperma pyrifolium in reducing carrageenan-induced inflammation and the inflammation induced by T. serrulatus envenomation in mice. We also evaluated the cytotoxic effects of this extract, using the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl-2H-tetrazolium (MTT) assay and the results revealed that the extract is safe. Analysis by High Performance Liquid Chromatography coupled with Diode Array Detector (HPLC-DAD) and Liquid Chromatography Coupled with Mass Spectrometry with Diode Array Detection (LC-DAD-MS) showed one major chemical component, the flavonoid rutin and phenolics compounds. For in vivo studies in carrageenan-induced peritonitis model, mice received extracts, dexamethasone, rutin or saline, before administration of carrageenan. For venom-induced inflammation model, animals received T. serrulatus venom and were, simultaneously, treated with extracts, antivenom, rutin or saline. The extract and rutin showed a reduction in the cell migration into the peritoneal cavity, and in the same way the envenomated animals also showed reduction of edema, inflammatory cell infiltration and vasodilation in lungs. This is an original study revealing the potential action of A. pyrifolium against inflammation caused by Tityus serrulatus venom and carrageenan, revealing that this extract and its bioactive molecules, specifically rutin, may present potential anti-inflammatory application. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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