Arabidopsis ACTIN-DEPOLYMERIZING FACTOR3 Is Required for Controlling Aphid Feeding from the Phloem.

Autor:	Mondal HA; Department of Biological Sciences, University of North Texas, Denton, Texas 76203.; Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar 736165, India., Louis J; Department of Biological Sciences, University of North Texas, Denton, Texas 76203.; Department of Entomology and Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68583., Archer L; Department of Biological Sciences, University of North Texas, Denton, Texas 76203.; BioDiscovery Institute, University of North Texas, Denton, Texas 76203., Patel M; Department of Biological Sciences, University of North Texas, Denton, Texas 76203.; BioDiscovery Institute, University of North Texas, Denton, Texas 76203., Nalam VJ; Department of Biological Sciences, University of North Texas, Denton, Texas 76203.; Department of Biology, Indiana University-Purdue University, Fort Wayne, Indiana 46805., Sarowar S; Department of Biological Sciences, University of North Texas, Denton, Texas 76203., Sivapalan V; Department of Biological Sciences, University of North Texas, Denton, Texas 76203., Root DD; Department of Biological Sciences, University of North Texas, Denton, Texas 76203., Shah J; Department of Biological Sciences, University of North Texas, Denton, Texas 76203 jyoti.shah@unt.edu.; BioDiscovery Institute, University of North Texas, Denton, Texas 76203.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2018 Jan; Vol. 176 (1), pp. 879-890. Date of Electronic Publication: 2017 Nov 13.
DOI:	10.1104/pp.17.01438
Abstrakt:	The actin cytoskeleton network has an important role in plant cell growth, division, and stress response. Actin-depolymerizing factors (ADFs) are a group of actin-binding proteins that contribute to reorganization of the actin network. Here, we show that the Arabidopsis ( Arabidopsis thaliana ) ADF3 is required in the phloem for controlling infestation by Myzus persicae Sülzer, commonly known as the green peach aphid (GPA), which is an important phloem sap-consuming pest of more than fifty plant families. In agreement with a role for the actin-depolymerizing function of ADF3 in defense against the GPA, we show that resistance in adf3 was restored by overexpression of the related ADF4 and the actin cytoskeleton destabilizers, cytochalasin D and latrunculin B. Electrical monitoring of the GPA feeding behavior indicates that the GPA stylets found sieve elements faster when feeding on the adf3 mutant compared to the wild-type plant. In addition, once they found the sieve elements, the GPA fed for a more prolonged period from sieve elements of adf3 compared to the wild-type plant. The longer feeding period correlated with an increase in fecundity and population size of the GPA and a parallel reduction in callose deposition in the adf3 mutant. The adf3 -conferred susceptibility to GPA was overcome by expression of the ADF3 coding sequence from the phloem-specific SUC2 promoter, thus confirming the importance of ADF3 function in the phloem. We further demonstrate that the ADF3 -dependent defense mechanism is linked to the transcriptional up-regulation of PHYTOALEXIN-DEFICIENT4 , which is an important regulator of defenses against the GPA. (© 2018 American Society of Plant Biologists. All Rights Reserved.)
Databáze:	MEDLINE
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