Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo.

Autor: Hultqvist LD; Costerton Biofilm Centre, Department of Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark., Alhede M; Costerton Biofilm Centre, Department of Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark., Jakobsen TH; Costerton Biofilm Centre, Department of Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark., Givskov M; Costerton Biofilm Centre, Department of Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark.; Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore., Bjarnsholt T; Costerton Biofilm Centre, Department of Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark. tbjarnsholt@sund.ku.dk.; Department of Clinical Microbiology, Rigshospitalet, 2100, Copenhagen, Denmark. tbjarnsholt@sund.ku.dk.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1673, pp. 203-212.
DOI: 10.1007/978-1-4939-7309-5_16
Abstrakt: In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.
Databáze: MEDLINE
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