The triolein/aqueous interface and lipase activity studied by spectroscopic ellipsometry and coarse grained simulations.

Autor: Stamm A; Physical Chemistry, Department of Chemistry, Lund University, P.O. Box 124, S-221 00 Lund, Sweden; Novozymes A/S, Brudelysvej 26, DK-2880 Bagværd, Denmark., Svendsen A; Novozymes A/S, Brudelysvej 26, DK-2880 Bagværd, Denmark., Skjold-Jørgensen J; Novozymes A/S, Brudelysvej 26, DK-2880 Bagværd, Denmark., Vissing T; Novozymes A/S, Brudelysvej 26, DK-2880 Bagværd, Denmark., Berts I; Jülich Centre for Neutron Science (JCNS), Forschungszentrum Jülich GmbH, Lichtenbergstr. 1, 85747 Garching, Germany., Nylander T; Physical Chemistry, Department of Chemistry, Lund University, P.O. Box 124, S-221 00 Lund, Sweden. Electronic address: Tommy.Nylander@fkem1.lu.se.
Jazyk: angličtina
Zdroj: Chemistry and physics of lipids [Chem Phys Lipids] 2018 Mar; Vol. 211, pp. 37-43. Date of Electronic Publication: 2017 Nov 10.
DOI: 10.1016/j.chemphyslip.2017.10.011
Abstrakt: In spite of the importance of the triglyceride aqueous interface for processes like emulsification, surfactant interactions and lipase activity, relatively little is known about this interface compared to that between alkanes and water. Here, the contact between triolein and water was investigated in terms of water inclusion in the oil phase and orientation of the molecules at the interface. Coarse grained models of triglycerides in contact with water were constructed and correlated with experimental results of the changes in thickness and refractive index, obtained using spectroscopic ellipsometry of spin-coated triolein films. The topography of the layer was revealed by atomic force microscopy. Dry triolein and a triolein sample after equilibration with water were also compared structurally using small-angle X-ray scattering. Additionally, the kinetics of adsorption/activity of three different variants of the Thermomyces lanuginosus lipase (TLL) were investigated. The results show that uptake of water in the triolein phase leads to increase in thickness of the layer. The observed increase of thickness was further enhanced by an active lipase but reduced when an inactive mutant of the enzyme was applied.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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