Coupling astogenic aging in the colonial tunicate Botryllus schlosseri with the stress protein mortalin.

Autor: Ben-Hamo O; Israel Oceanographic and Limnological Research, National Institute of Oceanography, Tel Shikmona, P.O. Box 8030, Haifa 31080, Israel; Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, 31905 Haifa, Israel. Electronic address: oshrat.bio@gmail.com., Rosner A; Israel Oceanographic and Limnological Research, National Institute of Oceanography, Tel Shikmona, P.O. Box 8030, Haifa 31080, Israel., Rabinowitz C; Israel Oceanographic and Limnological Research, National Institute of Oceanography, Tel Shikmona, P.O. Box 8030, Haifa 31080, Israel., Oren M; Israel Oceanographic and Limnological Research, National Institute of Oceanography, Tel Shikmona, P.O. Box 8030, Haifa 31080, Israel; Department of Molecular Biology, Ariel University, Ariel 40700, Israel., Rinkevich B; Israel Oceanographic and Limnological Research, National Institute of Oceanography, Tel Shikmona, P.O. Box 8030, Haifa 31080, Israel. Electronic address: buki@ocean.org.il.
Jazyk: angličtina
Zdroj: Developmental biology [Dev Biol] 2018 Jan 01; Vol. 433 (1), pp. 33-46. Date of Electronic Publication: 2017 Nov 08.
DOI: 10.1016/j.ydbio.2017.10.023
Abstrakt: Botryllus schlosseri, a colonial marine invertebrate, exhibits three generations of short-lived astogenic modules that continuously grow and die throughout the colony's entire lifespan, within week-long repeating budding cycles (blastogenesis), each consisting of four stages (A-D). At stage D, aging is followed by the complete absorption of adult modules (zooids) via a massive apoptotic process. Here we studied in Botryllus the protein mortalin (HSP70s member), a molecule largely known for its association with aging and proliferation. In-situ hybridization and qPCR assays reveal that mortalin follows the cyclic pattern of blastogenesis. Colonies at blastogenic stage D display the highest mortalin levels, and young modules exhibit elevated mortalin levels compared to old modules. Manipulations of mortalin with the specific allosteric inhibitor MKT-077 has led to a decrease in the modules' growth rate and the development of abnormal somatic/germinal morphologies (primarily in vasculature and in organs such as the endostyle, the stomach and gonads). We therefore propose that mortalin plays a significant role in the astogeny and aging of colonial modules in B. schlosseri, by direct involvement in the regulation of blastogenesis.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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