Differences in bacterial composition between men's and women's restrooms and other common areas within a public building.

Autor: Dobbler PCT; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa (UNIPAMPA), Avenida Antônio Trilha 1847, São Gabriel, Rio Grande do Sul, 97300-000, Brazil., Laureano ÁM; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa (UNIPAMPA), Avenida Antônio Trilha 1847, São Gabriel, Rio Grande do Sul, 97300-000, Brazil., Sarzi DS; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa (UNIPAMPA), Avenida Antônio Trilha 1847, São Gabriel, Rio Grande do Sul, 97300-000, Brazil., Cañón ERP; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa (UNIPAMPA), Avenida Antônio Trilha 1847, São Gabriel, Rio Grande do Sul, 97300-000, Brazil., Metz GF; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa (UNIPAMPA), Avenida Antônio Trilha 1847, São Gabriel, Rio Grande do Sul, 97300-000, Brazil., de Freitas AS; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa (UNIPAMPA), Avenida Antônio Trilha 1847, São Gabriel, Rio Grande do Sul, 97300-000, Brazil., Takagaki BM; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa (UNIPAMPA), Avenida Antônio Trilha 1847, São Gabriel, Rio Grande do Sul, 97300-000, Brazil., D Oliveira CB; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa (UNIPAMPA), Avenida Antônio Trilha 1847, São Gabriel, Rio Grande do Sul, 97300-000, Brazil., Pylro VS; Soil Microbiology Laboratory, Department of Soil Science, Luiz de Queiroz' College of Agriculture, University of São Paulo - ESALQ/USP, Av Pádua Dias, 11 CP 09, Piracicaba, São Paulo, 13400-970, Brazil., Copetti AC; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa (UNIPAMPA), Avenida Antônio Trilha 1847, São Gabriel, Rio Grande do Sul, 97300-000, Brazil., Victoria F; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa (UNIPAMPA), Avenida Antônio Trilha 1847, São Gabriel, Rio Grande do Sul, 97300-000, Brazil., Redmile-Gordon M; Department of Sustainable Soils and Grassland Systems, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK., Morais DK; Biosystems Informatics and Genomics Group, René Rachou Research Center, Belo Horizonte, Brazil., Roesch LFW; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa (UNIPAMPA), Avenida Antônio Trilha 1847, São Gabriel, Rio Grande do Sul, 97300-000, Brazil. luizroesch@unipampa.edu.br.
Jazyk: angličtina
Zdroj: Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2018 Apr; Vol. 111 (4), pp. 551-561. Date of Electronic Publication: 2017 Nov 10.
DOI: 10.1007/s10482-017-0976-6
Abstrakt: Humans distribute a wide range of microorganisms around building interiors, and some of these are potentially pathogenic. Recent research established that humans are the main drivers of the indoor microbiome and up to now significant literature has been produced about this topic. Here we analyzed differences in bacterial composition between men's and women's restrooms and other common areas within the same public building. Bacterial DNA samples were collected from restrooms and halls of a three-floor building from the Federal University of Pampa, RS, Brazil. The bacterial community was characterized by amplification of the V4 region of the 16S rRNA gene and sequencing. Throughout all samples, the most abundant phylum was Proteobacteria, followed by Actinobacteria, Bacteroidetes and Firmicutes. Beta diversity metrics showed that the structure of the bacterial communities were different among the areas and floors tested, however, only 6-9% of the variation in bacterial communities was explained by the area and floors sampled. A few microorganisms showed significantly differential abundance between men's and women's restrooms, but in general, the bacterial communities from both places were very similar. Finally, significant differences among the microbial community profile from different floors were reported, suggesting that the type of use and occupant demographic within the building may directly influence bacterial dispersion and establishment.
Databáze: MEDLINE
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