| Autor: |
Palovaara J; Laboratory of Biochemistry, Wageningen University, 6708 WE, Wageningen, The Netherlands., Saiga S; Laboratory of Biochemistry, Wageningen University, 6708 WE, Wageningen, The Netherlands., Wendrich JR; Laboratory of Biochemistry, Wageningen University, 6708 WE, Wageningen, The Netherlands.; Department of Plant Biotechnology and Bioinformatics and VIB Center for Plant Systems Biology, Ghent University, Technologiepark 927, 9052, Ghent, Belgium., van 't Wout Hofland N; Laboratory of Biochemistry, Wageningen University, 6708 WE, Wageningen, The Netherlands., van Schayck JP; Laboratory of Biochemistry, Wageningen University, 6708 WE, Wageningen, The Netherlands., Hater F; Laboratory of Biochemistry, Wageningen University, 6708 WE, Wageningen, The Netherlands., Mutte S; Laboratory of Biochemistry, Wageningen University, 6708 WE, Wageningen, The Netherlands., Sjollema J; Laboratory of Biochemistry, Wageningen University, 6708 WE, Wageningen, The Netherlands., Boekschoten M; Nutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen University, 6708 WE, Wageningen, The Netherlands., Hooiveld GJ; Nutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen University, 6708 WE, Wageningen, The Netherlands., Weijers D; Laboratory of Biochemistry, Wageningen University, 6708 WE, Wageningen, The Netherlands. dolf.weijers@wur.nl. |
| Abstrakt: |
During early plant embryogenesis, precursors for all major tissues and stem cells are formed. While several components of the regulatory framework are known, how cell fates are instructed by genome-wide transcriptional activity remains unanswered-in part because of difficulties in capturing transcriptome changes at cellular resolution. Here, we have adapted a two-component transgenic labelling system to purify cell-type-specific nuclear RNA and generate a transcriptome atlas of early Arabidopsis embryo development, with a focus on root stem cell niche formation. We validated the dataset through gene expression analysis, and show that gene activity shifts in a spatio-temporal manner, probably signifying transcriptional reprogramming, to induce developmental processes reflecting cell states and state transitions. This atlas provides the most comprehensive tissue- and cell-specific description of genome-wide gene activity in the early plant embryo, and serves as a valuable resource for understanding the genetic control of early plant development. |
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