| Autor: |
de Souza ÉMS; Programa de Pós-graduação em Genética, Conservação e Biologia Evolutiva, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Aleixo, 69.060-001 Manaus, AM, Brazil.; Laboratório de Genética Animal, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Aleixo, 69.060-001 Manaus, AM, Brazil., Gross MC; Universidade Federal da Integração Latino Americana, Laboratório de Genética, Av. Tarquínio Joslin dos Santos, 1000, Jardim Universitário, 85857-190, Foz do Iguaçu, PR, Brazil., Silva CEFE; Programa de Pós-graduação em Genética, Conservação e Biologia Evolutiva, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Aleixo, 69.060-001 Manaus, AM, Brazil.; Laboratório de Genética Animal, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Aleixo, 69.060-001 Manaus, AM, Brazil., Sotero-Caio CG; Department of Biological Sciences, Texas Tech University, Lubbock, TX, USA 79409.; Laboratório de Genética e Citogenética Animal e Humana, Departamento de Genética, Universidade Federal de Pernambuco, Av. da Engenharia s/n; Cidade Universitária; CEP:50740-600; Recife-PE, Brazil., Feldberg E; Laboratório de Genética Animal, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Aleixo, 69.060-001 Manaus, AM, Brazil. |
| Abstrakt: |
Species in the subgenus Artibeus Leach, 1821 are widely distributed in Brazil. Conserved karyotypes characterize the group with identical diploid number and chromosome morphology. Recent studies suggested that the heterochromatin distribution and accumulation patterns can vary among species. In order to assess whether variation can also occur within species, we have analyzed the chromosomal distribution of constitutive heterochromatin in A. planirostris (Spix, 1823) and A. lituratus (Olfers, 1818) from Central Amazon (North Brazil) and contrasted our findings with those reported for other localities in Brazil. In addition, Ag-NOR staining and FISH with 18S rDNA, telomeric, and LINE-1 probes were performed to assess the potential role that these different repetitive markers had in shaping the current architecture of heterochromatic regions. Both species presented interindividual variation of constitutive heterochromatin. In addition, in A. planirostris the centromeres of most chromosomes are enriched with LINE-1, colocated with pericentromeric heterochromatin blocks. Overall, our data indicate that amplification and differential distribution of the investigated repetitive DNAs might have played a significant role in shaping the chromosome architecture of the subgenus Artibeus. |
