Clinical and environmental isolates of Burkholderia pseudomallei from Brazil: Genotyping and detection of virulence gene.

Autor:	Bandeira TJPG; Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil; Christus School of Medicine, Fortaleza, Ceará, Brazil., Castelo-Branco DSCM; Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil., Rocha MFG; Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil; Postgraduate Program in Veterinary Sciences, State University of Ceará, Fortaleza, Ceará, Brazil., Cordeiro RA; Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil., Ocadaque CJ; Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil., Paiva MAN; Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil; Federal Institute of Education, Science and Technology of Ceará, Acaraú, Ceará, Brazil., Brilhante RSN; Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: brilhante@ufc.br., Sidrim JJC; Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Jazyk:	angličtina
Zdroj:	Asian Pacific journal of tropical medicine [Asian Pac J Trop Med] 2017 Oct; Vol. 10 (10), pp. 945-951. Date of Electronic Publication: 2017 Sep 18.
DOI:	10.1016/j.apjtm.2017.09.004
Abstrakt:	Objective: To evaluate the genetic diversity of clinical and environmental isolates of Burkholderia pseudomallei (B. pseudomallei) recovered in Ceará, Brazil, and screen these isolates for the presence of type three secretion system virulence gene. Methods: Nineteen B. pseudomallei isolates (9 from clinical cases and 10 from soils) were analyzed. Random amplified polymorphic DNA was performed with primers OPQ-2, OPQ-4 and OPQ-16 to evaluate the genetic diversity, and type three secretion system gene was detected through polymerase chain reaction. Results: Random amplified polymorphic DNA showed a genetic relatedness of approximately 50% among the tested B. pseudomallei isolates, which were grouped into two clades, of which the biggest ones comprised 18/19 isolates for primer OPQ-2, and 17/19 isolates for primer OPQ-16. Primer OPQ-4 grouped the isolates into three clades comprising 1/19, 3/19 and 15/19 isolates. Additionally, type three secretion system gene was detected in all tested isolates. Conclusions: This is an effort to type B. pseudomallei strains from Ceará, which is important for better understanding this pathogen, contributing for the epidemiological surveillance of melioidosis in this endemic region. (Copyright © 2017 Hainan Medical University. Production and hosting by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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