Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix.

Autor: Ramírez-Tapias YA; Laboratory of Sustainable Biotechnology (LIBioS), National University of Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Argentina.; National Scientific and Technical Research Council (CONICET), Godoy Cruz 2290, C1425FQB Cuidad Autónoma de Buenos Aires (CABA), Buenos Aires Argentina., Lapasset Laumann AS; Laboratory of Sustainable Biotechnology (LIBioS), National University of Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Argentina., Britos CN; Laboratory of Sustainable Biotechnology (LIBioS), National University of Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Argentina., Rivero CW; Laboratory of Sustainable Biotechnology (LIBioS), National University of Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Argentina.; National Scientific and Technical Research Council (CONICET), Godoy Cruz 2290, C1425FQB Cuidad Autónoma de Buenos Aires (CABA), Buenos Aires Argentina., Trelles JA; Laboratory of Sustainable Biotechnology (LIBioS), National University of Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Argentina.; National Scientific and Technical Research Council (CONICET), Godoy Cruz 2290, C1425FQB Cuidad Autónoma de Buenos Aires (CABA), Buenos Aires Argentina.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2017 Dec; Vol. 7 (6), pp. 380. Date of Electronic Publication: 2017 Oct 26.
DOI: 10.1007/s13205-017-1010-4
Abstrakt: Enzyme immobilization using hydrogels is a low-cost and effective system for the degradation of bulk pectin derived from orange industry residues. Polygalacturonases obtained from four different bacterial strains of Streptomyces genus were immobilized in alginate gel and assayed for pectin hydrolysis. The enzyme from Streptomyces halstedii ATCC 10897 proved to be superior and more stable within the alginate matrix. Furthermore, a new strategy to improve alginate bead stability using a mixture of calcium and strontium is reported; this technique allowed enhancing the mechanical properties by combining different amounts of these cations for ionotropic gelation. The developed biocatalyst showed maximum hydrolysis at 2 h, generating 1.54 mg/mL of reducing sugars and decreasing the viscosity of polygalacturonic acid by 98.9%. Reusability up to 29 successive reactions (58 h) demonstrated a very stable performance. The heterogeneous biocatalyst was used in the enzymatic saccharification of orange peel albedo (2.23 mg/mL) for adding value to this agro-waste by industrial exploitation.
Databáze: MEDLINE