Layer-by-layer assembly of graphene oxide on thermosensitive liposomes for photo-chemotherapy.

Autor: Hashemi M; Department of Biomedical Engineering, Faculty of New Sciences and Technologies, The University of Tehran, Tehran, Iran; Department of Biomedical Engineering, The University of Texas at Austin, Austin, USA; Division of Pharmaceutics, College of Pharmacy, The University of Texas at Austin, Austin, USA., Omidi M; Protein Research Center, Shahid Beheshti University, GC, Velenjak, Tehran, Iran., Muralidharan B; Department of Biomedical Engineering, The University of Texas at Austin, Austin, USA; Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, USA., Tayebi L; Department of Developmental Sciences, Marquette University School of Dentistry, Milwaukee, USA., Herpin MJ; Division of Pharmaceutics, College of Pharmacy, The University of Texas at Austin, Austin, USA., Mohagheghi MA; Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran., Mohammadi J; Department of Biomedical Engineering, Faculty of New Sciences and Technologies, The University of Tehran, Tehran, Iran. Electronic address: hashemi@utexas.edu., Smyth HDC; Division of Pharmaceutics, College of Pharmacy, The University of Texas at Austin, Austin, USA. Electronic address: hugh.smyth@austin.utexas.edu., Milner TE; Department of Biomedical Engineering, The University of Texas at Austin, Austin, USA. Electronic address: tmilner@utexas.edu.
Jazyk: angličtina
Zdroj: Acta biomaterialia [Acta Biomater] 2018 Jan; Vol. 65, pp. 376-392. Date of Electronic Publication: 2017 Nov 08.
DOI: 10.1016/j.actbio.2017.10.040
Abstrakt: Stimuli responsive polyelectrolyte nanoparticles have been developed for chemo-photothermal destruction of breast cancer cells. This novel system, called layer by layer Lipo-graph (LBL Lipo-graph), is composed of alternate layers of graphene oxide (GO) and graphene oxide conjugated poly (l-lysine) (GO-PLL) deposited on cationic liposomes encapsulating doxorubicin. Various concentrations of GO and GO-PLL were examined and the optimal LBL Lipo-graph was found to have a particle size of 267.9 ± 13 nm, zeta potential of +43.9 ± 6.9 mV and encapsulation efficiency of 86.4 ± 4.7%. The morphology of LBL Lipo-graph was examined by cryogenic-transmission electron microscopy (Cryo-TEM), atomic force microcopy (AFM) and scanning electron microscopy (SEM). The buildup of LBL Lipo-graph was confirmed via ultraviolet-visible (UV-Vis) spectrophotometry, thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analysis. Infra-red (IR) response suggests that four layers are sufficient to induce a gel-to-liquid phase transition in response to near infra-red (NIR) laser irradiation. Light-matter interaction of LBL Lipo-graph was studied by calculating the absorption cross section in the frequency domain by utilizing Fourier analysis. Drug release assay indicates that the LBL Lipo-graph releases much faster in an acidic environment than a liposome control. A cytotoxicity assay was conducted to prove the efficacy of LBL Lipo-graph to destroy MD-MB-231 cells in response to NIR laser emission. Also, image stream flow cytometry and two photon microcopy provide supportive data for the potential application of LBL Lipo-graph for photothermal therapy. Study results suggest the novel dual-sensitive nanoparticles allow intracellular doxorubin delivery and respond to either acidic environments or NIR excitation.
Statement of Significance: Stimuli sensitive hybrid nanoparticles have been synthesized using a layer-by-layer technique and demonstrated for dual chemo-photothermal destruction of breast cancer cells. The hybrid nanoparticles are composed of alternating layers of graphene oxide and graphene oxide conjugated poly-l-lysine coating the surface of a thermosensitive cationic liposome containing doxorubicin as a core. Data suggests that the hybrid nanoparticles may offer many advantages for chemo-photothermal therapy. Advantages include a decrease of the initial burst release which may result in the reduction in systemic toxicity, increase in pH responsivity around the tumor environment and improved NIR light absorption.
(Copyright © 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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