Boundary Formation through a Direct Threshold-Based Readout of Mobile Small RNA Gradients.

Autor:	Skopelitis DS; Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA., Benkovics AH; Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA., Husbands AY; Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA., Timmermans MCP; Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA; Center for Plant Molecular Biology, University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany. Electronic address: marja.timmermans@zmbp.uni-tuebingen.de.
Jazyk:	angličtina
Zdroj:	Developmental cell [Dev Cell] 2017 Nov 06; Vol. 43 (3), pp. 265-273.e6. Date of Electronic Publication: 2017 Oct 26.
DOI:	10.1016/j.devcel.2017.10.003
Abstrakt:	Small RNAs have emerged as a new class of mobile signals. Here, we investigate their mechanism of action and show that mobile small RNAs generate sharply defined domains of target gene expression through an intrinsic and direct threshold-based readout of their mobility gradients. This readout is highly sensitive to small RNA levels at the source, allowing plasticity in the positioning of a target gene expression boundary. Besides patterning their immediate targets, the readouts of opposing small RNA gradients enable specification of robust, uniformly positioned developmental boundaries. These patterning properties of small RNAs are reminiscent of those of animal morphogens. However, their mode of action and the intrinsic nature of their gradients distinguish mobile small RNAs from classical morphogens and present a unique direct mechanism through which to relay positional information. Mobile small RNAs and their targets thus emerge as highly portable, evolutionarily tractable regulatory modules through which to create pattern. (Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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