Single nucleotide polymorphism rs2274084 of gap junction protein beta 2 gene among Epstein-Barr virus-associated tumors.

Autor: Xiao H; Department of Medical Microbiology, Qingdao University Medical College, Qingdao 266021, Shandong, China., Liu W; Department of Medical Microbiology, Qingdao University Medical College, Qingdao 266021, Shandong, China., Zhao Z; Department of Medical Microbiology, Qingdao University Medical College, Qingdao 266021, Shandong, China., Zhang Y; Department of Medical Microbiology, Qingdao University Medical College, Qingdao 266021, Shandong, China.; Department of Clinical Laboratory, Central Hospital of Zibo, Zibo 255036, Shandong, China., Song Y; Department of Medical Microbiology, Qingdao University Medical College, Qingdao 266021, Shandong, China., Luo B; Department of Medical Microbiology, Qingdao University Medical College, Qingdao 266021, Shandong, China.
Jazyk: angličtina
Zdroj: Cancer biomarkers : section A of Disease markers [Cancer Biomark] 2018 Feb 14; Vol. 21 (3), pp. 499-504.
DOI: 10.3233/CBM-170078
Abstrakt: Background: Gap junction protein beta 2 gene (GJB2) encodes one of connexins- Connexin 26 (Cx26), which mainly expressed in epithelial cells. Cx26 is usually considered a channel to exchange information between cells, which plays a critical role in tumor cell proliferation.
Objective: We investigated GJB2 rs2274084 polymorphism in three types of tumors, including nasophoryngeal carcinoma (NPC), gastric cancer (GC) and lymphoma.
Methods: Proteinase K digestion and phenolchloroform purification and QIAamp DNA FFPE tissue kit was used for DNA extraction. The genotype of GJB2 gene rs2274084 was detected through Sequenom MassARRAY SNP technique. The Chi-square test and Fisher's exact test were used to compare the differences between two groups.
Results: The genotype frequency of GJB2 gene rs2274084 was significantly different between EBV-positive NPC and normal control (P< 0.05). However, for EBV-associated gastric cancer (EBVaGC), EBV-negative gastric cancer (EBVnGC) and lymphoma, no significant differences were found in comparison with the normal control.
Conclusions: The mutation rate of TT genotype was a risk factor to the occurrence of EBV-positive NPC.
Databáze: MEDLINE
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