Genome Analysis and Genetic Stability of the Cryptophlebia leucotreta Granulovirus (CrleGV-SA) after 15 Years of Commercial Use as a Biopesticide.

Autor: van der Merwe M; Department of Biochemistry and Microbiology, P.O. Box 94, Rhodes University, Grahamstown 6140, South Africa. vandermerwe_marcel@yahoo.com., Jukes MD; Department of Biochemistry and Microbiology, P.O. Box 94, Rhodes University, Grahamstown 6140, South Africa. mrmdjukes@gmail.com., Rabalski L; Department of Recombinant Vaccines, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, 80-210 Gdansk, Poland. lukasz.rabalski@biotech.ug.edu.pl., Knox C; Department of Biochemistry and Microbiology, P.O. Box 94, Rhodes University, Grahamstown 6140, South Africa. caroline.knox@ru.ac.za., Opoku-Debrah JK; River Bioscience, P.O. Box 20388, Humewood, Port Elizabeth 6013, South Africa. john@riverbio.com., Moore SD; Citrus Research International, P.O. Box 20285, Humewood, Port Elizabeth 6013, South Africa. seanmoore@cri.co.za.; Department of Zoology and Entomology, P.O. Box 94, Rhodes University, Grahamstown 6140, South Africa. seanmoore@cri.co.za., Krejmer-Rabalska M; Department of Recombinant Vaccines, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, 80-210 Gdansk, Poland. martyna.krejmer@biotech.ug.edu.pl., Szewczyk B; Department of Recombinant Vaccines, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, 80-210 Gdansk, Poland. boguslaw.szewczyk@biotech.ug.edu.pl., Hill MP; Department of Zoology and Entomology, P.O. Box 94, Rhodes University, Grahamstown 6140, South Africa. m.hill@ru.ac.za.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2017 Nov 03; Vol. 18 (11). Date of Electronic Publication: 2017 Nov 03.
DOI: 10.3390/ijms18112327
Abstrakt: Thaumatotibia leucotreta Meyrick (Lepidoptera: Tortricidae) is an indigenous pest in southern Africa which attacks citrus fruits and other crops. To control T. leucotreta in South Africa, an integrated pest management (IPM) programme incorporating the baculovirus Cryptophlebia leucotreta granulovirus (CrleGV-SA) as a biopesticide has been implemented. This study investigated the genetic stability of a commercially produced CrleGV-SA product that has been applied in the field since 2000. Seven representative full-genome sequences of the CrleGV-SA isolate spanning a 15-year period were generated and compared with one another. Several open reading frames (ORFs) were identified to have acquired single nucleotide polymorphisms (SNPs) during the 15-year period, with three patterns observed and referred to as "stable", "reversion", and "unstable switching". Three insertion events were also identified, two of which occurred within ORFs. Pairwise multiple alignments of these sequences showed an identity ranging from 99.98% to 99.99%. Concentration-response bioassays comparing samples of CrleGV-SA from 2000 and 2015 showed an increase in virulence toward neonate T. leucotreta larvae. The CrleGV-SA genome sequence generated from the 2015 sample was compared to the Cape Verde reference genome, CrleGV-CV3. Several fusion events were identified between ORFs within these genomes. These sequences shared 96.7% pairwise identity, confirming that CrleGV-SA is a genetically distinct isolate. The results of this study indicate that the genome of CrleGV-SA has remained stable over many years, with implications for its continued use as a biopesticide in the field. Furthermore, the study describes the first complete baculovirus genome to be sequenced with the MinION (Oxford Nanopore, Oxford, UK) platform and the first complete genome sequence of the South African CrleGV isolate.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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