[Difference of chemical constituents in Eucommiae Cortex from different habitats by LC-QTOF MS/MS].

Autor:	Yan Y; School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China., Zhao H; School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China., Zou LS; School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China., Liu XH; School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China., Chai C; School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China., Wang SN; School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China., Hua YJ; School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Jazyk:	čínština
Zdroj:	Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2017 Jul; Vol. 42 (14), pp. 2730-2737.
DOI:	10.19540/j.cnki.cjcmm.20170419.004
Abstrakt:	In order to study the influence of ecological environment regarding the synthesis and accumulation of metabolites in Eucommiae Cortex, LC-QTOF MS/MS method combined with multivariate statistical analysis was used to analyze the differences of chemical constituents in Eucommiae Cortex from different habitats. Through the analysis of the multistage tandem mass spectrometry, the characteristic peaks were extracted with mass spectrometry data peak matching, peak alignment, and noise filtering. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used for data processing. The chemical constituents were identified or tentative presumed according to MS accurate mass and MS/MS spectrometry fragmentation information, combined with the software of database search, comparison with reference standards and literature. The results show the differences among samples of Eucommiae Cortex from different habitats are distinguishable. A total of 23 chemical constituents in Eucommiae Cortex were identified or tentative presumed. Among of them, 14 kinds of common differential chemical constituents (aucubin, geniposidic acid, neochlorogenic acid, syringin, olivil-4',4'-di-O-β-D-glucopyranoside, chlorogenic acid, cryptochlorogenic acid, 1-hydroxypinoresinol- 4',4'-di-O-β-D-glucopyranoside, caffeic acid, pinoresinol-di-O-β-D-glucopyranoside, syringaresionl-di-O-β-D-glucopyranoside, pinoresinol-4'-O-β-D-glucopyranoside, eucommiol, isochlorogenic acid C and asiatic acid) presented different changing laws. This study provides basic information for revealing the influence law of ecological environment on the biosynthesis of metabolites in Eucommiae Cortex. Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose. (Copyright© by the Chinese Pharmaceutical Association.)
Databáze:	MEDLINE
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