Casticin impairs cell growth and induces cell apoptosis via cell cycle arrest in human oral cancer SCC-4 cells.

Autor: Chou GL; Department of Biological Science and Technology, China Medical University, Taichung, 404, Taiwan., Peng SF; Department of Biological Science and Technology, China Medical University, Taichung, 404, Taiwan., Liao CL; Graduate Institute of Chinese Medicine, China Medical University, Taichung, 404, Taiwan., Ho HC; School of Medicine, China Medical University, Taichung, 404, Taiwan., Lu KW; College of Chinese Medicine, School of Post-Baccalaureate Chinese Medicine, China Medical University, Taichung, 404, Taiwan., Lien JC; School of Pharmacy, China Medical University, Taichung, 404, Taiwan., Fan MJ; Department of Biotechnology, Asia University, Taichung, 413, Taiwan., La KC; Department of Medical Laboratory Science and Biotechnology, College of Medicine and Life Science, Chung Hwa University of Medical Technology, Tainan, 717, Taiwan., Chung JG; Department of Biological Science and Technology, China Medical University, Taichung, 404, Taiwan.; Department of Biotechnology, Asia University, Taichung, 413, Taiwan.
Jazyk: angličtina
Zdroj: Environmental toxicology [Environ Toxicol] 2018 Feb; Vol. 33 (2), pp. 127-141. Date of Electronic Publication: 2017 Nov 03.
DOI: 10.1002/tox.22497
Abstrakt: Casticin, a polymethoxyflavone, present in natural plants, has been shown to have biological activities including anti-cancer activities. Herein, we investigated the anti-oral cancer activity of casticin on SCC-4 cells in vitro. Viable cells, cell cycle distribution, apoptotic cell death, reactive oxygen species (ROS) production, and Ca 2+ production, levels of ΔΨ m and caspase activity were measured by flow cytometric assay. Cell apoptosis associated protein expressions were examined by Western blotting and confocal laser microscopy. Results indicated that casticin induced cell morphological changes, DNA condensation and damage, decreased the total viable cells, induced G 2 /M phase arrest in SCC-4 cells. Casticin promoted ROS and Ca 2+ productions, decreases the levels of ΔΨ m , promoted caspase-3, -8, and -9 activities in SCC-4 cells. Western blotting assay demonstrated that casticin affect protein level associated with G2/M phase arrest and apoptosis. Confocal laser microscopy also confirmed that casticin increased the translocation of AIF and cytochrome c in SCC-4 cells. In conclusion, casticin decreased cell number through G 2 /M phase arrest and the induction of cell apoptosis through caspase- and mitochondria-dependent pathways in SCC-4 cells.
(© 2017 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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